Molecular epidemiological study of factor to fluctuate the level of contamination of Vibrio parahaemolyticus in water of fishing port and coastal waters

副溶血弧菌(BALO)裂解为一种河口生物--副溶血弧菌，广泛分布在沿海水域，因此流入大海的副溶血弧菌可能会被BALO杀死。在此基础上，分析了佐田港、幕津港及相关河口的BALO生态。在Murotsu河口生长的Balo和副溶血弧菌在降雨时飞出大海，飞到Murotsu港，但河口的生物量在10天内恢复。在萨达河口发现了嗜盐和微嗜盐的巴洛斯。副溶血弧菌在河口的含量与BALO的含量相关，但与近岸水域的BALO水平无关。在河口沉积物中生长的副溶血弧菌可能会流出到因上游降雨而涨水的近岸水域，污染鱼类和贝类，并造成食物中毒。2004年7月新泻县暴雨过后两周，新泻口岸检出副溶血性弧菌，数量在23~240条/g之间，随后呈下降趋势。荒川口水位上升后7~14d发生副溶血性弧菌-胃肠炎暴发。根据这些证据，在河口生长的副溶血性弧菌被证明流入海洋并污染那里的鱼类和贝类。

Bdellovibrio-and-like organisms (BALO) lytic to an estuarine organism, Vibrio parahaemolyticus, range in coastal waters so that V.parahaemolyticus flow out to the sea is suspected to be killed by BALO. Thus the ecology of BALO was analyzed at Sada and Murotsu Ports and related estuaries. BALO and V.parahaemolyticus grown in Murotsu Estuary flew out to the sea and flew into Murotsu Port at the rainfall, though the levels of the organisms at the estuary recovered within 10 days. Halophilic and micro-halophilic BALos were detected at Sada Estuary. Level of V.parahaemolyticus was correlated to that of BALO at estuaries but not at coastal waters.V.parahaemolyticus grown in estuarine sediments was suspected to flow out to coastal waters with water swollen by the rainfall at the upper streams, contaminate fishes and shellfishes and cause food poisonings. V.parahaemolyticus was detected from horse mackerels landed at Niigata Port at a level of 23〜240/g two weeks after the heavy rain on July 2004 at Niigata Prefecture, whose levels decreased afterwards. Outbreaks of V.parahaemolyticus-gastroenteritis happened after the consumption of oyster 7〜14 days after the rise of water level at Arakawa Estuary. From these evidences, V.parahaemolyticus grown at estuaries was demonstrated to flow into the sea and contaminate fishes and shellfishes there.