Study on easy diagnostic system for cervical cancer with using p16 antibody

据报道，在宫颈病变中，p16的过表达与高危人乳头瘤病毒感染密切相关（Am J Pathol，1998）。本研究的目的是证实液基宫颈标本与组织切片一样可用于p16染色。研究结果证实，使用液基方法，p16的免疫组化检测比宫颈病变中的HPV状态更敏感和特异，这表明p16应该用作宫颈细胞学初步筛查的令人满意的生物标志物（Cancer cytopathol，2004）。14 -3-3sigma一直是主要的G2/M检查点控制基因。为了证实14-3-3sigma蛋白与p16在宫颈癌中的表达，进行免疫组织化学。在所有宫颈不典型增生和癌包括腺癌中均观察到14-3- 3 σ的强和弥漫性免疫反应性。研究结果证实，大多数p16过表达的宫颈癌也表现出14-3-3sigma的过表达（Pathol Int，2004）。我们还研究了14-3-3sigma在子宫内膜腺癌和非肿瘤性子宫内膜中的表达，这两种情况都可能与宫颈病变一起通过细胞学标本获得。在正常子宫内膜中，14-3- 3 σ在分泌中期至晚期过度表达。在子宫内膜腺癌中，14-3-3sigma表达随着组织学分级、肌层浸润程度的增加而显著增加。这些结果表明，需要仔细注意宫颈标本中14-3- 3 σ的免疫组织化学表达的解释，包括正常或肿瘤性宫颈炎（Pathol Int，2005）。

In cervical lesions, the overexpression of p16 is reported to be closely associated with high-risk human papillomavirus infection (Am J Pathol, 1998). The objective of the current study was to confirm the usefulness of liquid-based cervical specimens for p16 staining wa well as tissue sections. The results of the study confirmed that immunohistochemical detection of p16 was more sensitive and specific than HPV status in cervical lesions using a liquid based method, suggesting that p16 should be used as a satisfactory biomarker for the primary screening of cervical cytology (Cancer cytopathol, 2004).14-3-3sigma has been a major G2/M checkpoint control gene. In order to confirm 14-3-3sigma protein expression together with p16 in cervical cancers, immunohistochemistry was performed. Strong and diffuse immunoreactivity for 14-3-3sigma was uniformly observed in all cervical dysplasia and carcinomas including adenocarcinomas. The results of the study confirmed that the majority of cervical cancers with p16 overexpression also showed overexpression of 14-3-3sigma (Pathol Int, 2004).We also investigated the expression of 14-3-3sigma in endometrial adenocarcinoma and non-neoplastic endometria, both which might be obtained together with cervical lesions by cytology specimens. In normal endometrium, 14-3-3sigma was overexpressed in the mid- to late- secretory phase. In endometrial adenocarcinoma, 14-3-3sigma expression increased significantly with increasing histological grade, myometrial invasion. These results suggest that careful attention is needed on the interpretation for immunohistochemical expression of 14-3-3sigma in cervical specimens including normal or neoplastic endometria (Pathol Int, 2005).