The influence of ghrelin pormorplism on the occurence of cardiovascular disease and gastric acid secretion

我们于2004年4月至2006年3月调查了ghrelin多态性对心血管疾病危险因素和胃酸分泌的影响。在获得书面知情同意书后，112例接受大规模筛查的患者入组本研究。目的多态位点为Arg 51 Gln（外显子2的G346 A）、Leu 72 Met（外显子2的C408 A）和内含子1的G274 A。在本研究受试者中，112例受试者均未出现G346 A和G274 A区域的突变基因。在C408 A区，我们发现41例受试者为异型C408 A突变，3例受试者为突变型/突变型。未观察到C408 A区域突变与幽门螺杆菌感染的关系。此外，C408 A区域的突变与血浆ghrelin浓度和血清总胆固醇、HDL-胆固醇和甘油三酯浓度无关。我们计划下一步研究C408 A区多态性与胃酸分泌的关系。我们阐明了在我们进行本研究期间，日本人群中非糜烂性胃食管反流病病例的患病率。动脉硬化程度与食管裂孔疝患病率之间的关系也被阐明。

We investigated the influence the ghrelin pormorphism on the risk factors of cardiovascular diseases and gastric acid secretion between April 2004 and March 2006. After obtaining written informed consent,112 cases, who visited for mass screening, were enrolled in this study. Target polymorphic sites were Arg51Gln (G346A in exon-2), Leu72Met (C408A in exon-2) and G274A in intron-1. None of the 112 subjects had mutant gene in G346A and G274A regions in this study subjects. In C408A region, we found 41 subjects with hetero-type C408A mutation and 3 subjects with mutant/mutant. The relationship between the mutation of C408A region and Helicobacter pylori infection was not observed. In addition, the mutation of C408A region was not correlated with the plasma ghrelin concentration and serum concentrations of total cholesterol, HDL-cholesterol, and triglyceride. We are planning to investigate the relationship between the pormorphism of C408A region and gastric acid secretion in next step. We clarified the prevalence of the cases with non-erosive gastro-esophageal reflux disease in Japanese population during we performing this study. The relationship between the degree of arteriosclerosis and the prevalence of hiatal hernia was also clarified.