T cell epitope of GAD antigen in type 1 diabetes

在获得庆应义塾大学医学院伦理委员会的知情同意和批准后，招募典型的1型糖尿病患者。选取年龄33岁、GAD抗体、病程短、HLA DR9的1型糖尿病患者。取患者外周血淋巴细胞，用重组人GAD抗原刺激，建立GAD反应性T细胞克隆。我们可以获得一个T细胞克隆，并证实克隆的表型为CD4，确实克隆对GAD抗原有特异性反应。我们现在用GAD肽刺激克隆来鉴定T细胞表位。

Typical type 1 diabetic patients were recruited after obtaining informed consent and approval of ethics committee of Keio Univ. School of Medicine. Among those patients, 33 year old type 1 diabetic patient with GAD antibody, short duration of disease, and HLA DR9 was selected. Peripheral blood lymphocytes were obtained from the patient, and those were stimulated with recombinant human GAD antigen to establish GAD reactive T cell clones. We could obtain a T cell clone, and confirmed that the phenotype of the clone is CD4, and indeed the clone reacts specifically to GAD antigen. We are now stimulating the clone with GAD peptide to identify the T cell epitopes.