Study for epileptogenesis arising from the neuronal networks among amygdala, entorhinal cortex and hippocampus.

近年来，内嗅皮层的变性在边缘癫痫的发病中起着重要的作用，内嗅皮层的纤维主要与海马体相连。我们用包含保持神经元连接的内嗅皮层的联合海马片研究了这一区域。到目前为止，内嗅皮层的癫痫发生机制已被我们以前的项目阐明。在本项目中，我们研究了抗癫痫药物对联合应用喹诺酮和氟比洛芬(分别为抗菌药物和止痛药)的海马片癫痫放电的抑制作用。抑制作用最强的是卡马西平，其次是苯巴比妥和丙戊酸。我们还开发了包括杏仁核在内的海马旁联合切片。在这些合并的海马区脑片中，有四分之一的脑片在没有任何药物处理的情况下被发现有周期性的振荡活动。神经网络被保存在这些脑片中，在手术分离这些脑片以切断海马区旁神经元网络后，识别这种活动的去同步化。

Recently, degeneration of entorhinal cortex, which fibers mostly connect to hippocampus proper, are playing an important role of epileptogenesis in limbic seizure. We have investigated this area with combined hippocampal slice which includes entorhinal cortices keeping neuronal connection. Thus far, the epileptogenesis of entorhinal cortices was elucidated by our former project.In this project, we investigated the inhibitory effect of antiepileptic drugs on epileptic discharges recorded from the combined hippocampal slices with bath application of Quinolone and Flurbiprofen (antibacterial drug and analgesic respectively). The strongest inhibition was shown by Carbamazepine, followed by Phenobarbital and Valproic Acid.We also developed combined parahippocampal slice including amygdala. Periodic oscillatory activity was identified in a quarter of these combined parahippocampal slices without any pharmacological manipulation. The neural networks were kept in these slices, identifying with de-synchronization of this activity after surgical separation of these slices to disconnect the parahippocampal neuronal networks.Teses combined hippocampal and parahippocampal slices are useful tools for analyzing the underlying mechanisms of limbic seizure.