Possible role of stathmin in the uterine function and in the mechanism of endometrial stroma decidualization

在围着床期小鼠子宫中检测stathmin及其家族基因的表达。在妊娠早期，stathmin主要定位于子宫内膜的腺上皮、腔上皮、血管和间质细胞。Stathmin的强表达仅限于毛细血管和次级蜕膜细胞。Stathmin的表达在着床部位高于着床部位之间的子宫段，并在油诱导的蜕膜化过程中增加。stathmin-KO小鼠的新生幼崽较少（减少30%）。碱性磷酸酶、结蛋白和细胞周期蛋白D3的表达在stathmin-KO小鼠的蜕膜区减弱。在WT和stathmin-KO小鼠中，stathmin家族基因SCG 10的mRNA水平在蜕膜化时较高。相反，与WT小鼠相比，SCLIP和RB 3在stathmin-KO小鼠中高表达。这些结果表明，stathmin和stathmin家族基因表达在细胞中， ...更多信息 小鼠子宫内膜在蜕膜化过程中表达增强。由于stathmin在人子宫中的表达尚未被研究，因此执行stathmin在人子宫中的功能。Stathmin表达于人子宫内膜组织的腺上皮和基质细胞，以及妊娠早期的细胞滋养细胞和绒毛外滋养细胞。当从正常子宫内膜分离的基质细胞培养和刺激蜕膜化卵巢类固醇或双丁酰环AMP，磷酸化stathmin水平逐渐下降，提示stathmin可能参与子宫内膜间质细胞的蜕膜化。然而，敲低stathmin在培养的基质细胞中的表达，通过使用siRNA细胞暴露于蜕膜化剂显着抑制蜕膜化，如IGF结合蛋白-1和催乳素的表达减少所示。Stathmin在人子宫内膜和胎盘细胞中表达差异，可能参与子宫内膜间质细胞的蜕膜化。少

The expression of stathmin and its family genes was examined in the murine uterus during the peri-implantation period. During the early stage of pregnancy, stathmin was mainly localized in the glandular and luminal epithelium, blood vessels, and stromal cells in the endometrium. Intense stathmin expression was limited to capillary vessels and secondary decidual cells. Stathmin expression was higher at implantation sites than at uterine segments between implantation sites and increased during oil-induced decidualization. The stathmin-KO mice had fewer newborn pups (reduced by 30%). The expression of alkaline phosphatase, desmin, and cyclin D3 was attenuated in decidual zones of stathmin-KO mice. Messenger RNA level of the stathmin family gene, SCG10, was high at the time of decidualization in WT and stathmin-KO mice.In contrast, SCLIP and RB3 were highly expressed in stathmin-KO mice compared to WT mice. These results suggest that stathmin and stathmin family genes are expressed in the … More murine endometrium with enhanced expression in the decidualization process. Because stathmin expression in the human uterus has not yet been investigated, the function of stathmin in human uterus was executed. Stathmin is expressed in the glandular epithelium and stromal cells of human endometrial tissue, and in cytotrophoblasts and extravillous trophoblasts during the first-trimester of pregnancy. When stromal cells isolated from normal endometrium were cultured and stimulated to decidualize by ovarian steroids or dibutyryl cyclic AMP, phosphorylated stathmin levels gradually decreased, suggesting stathmin may participate in endometrial stromal cell decidualization. However, knocking down stathmin expression in the cultured stromal cells by using siRNA before the cells were exposed to the decidualizing agents significantly suppressed decidualization, as indicated by the decreased expression of IGF-binding protein-1 and prolactin. Stathmin is differently expressed in human endometrial and placental cells and may be involved in the decidualization of endometrial stromal cells. Less