Establishment of hybrid type artificial bone using vascularized periosteal flap and PHAB

为了阐明实验性骨膜附着羟基磷灰石块孔内成骨的时序性变化，在兔模型上进行了详细的显微观察和孔的形态计量分析。在整个实验过程中，对照组的肌肉内植入的PHAB的孔隙中没有发现骨和脂肪组织。8周时，新生骨组织证实为层状结构和去极化，最初出现在靠近长方形PHAB骨膜覆盖的孔边，这种孔中的骨形成从被覆盖的面延伸到远端。12周时，在骨壁内可见脂肪组织排列于骨孔内。形态计量学分析显示，骨在每个孔洞中的面积百分比与PHAB附着骨膜表面的距离呈负相关，推测PHAB表面附着骨膜提供的间充质干细胞通过骨膜附着于PHAB表面，从而导致骨塌陷的形成，并由脂肪组织填充成红细胞的胰岛。我们要强调的是，所提供的PHAB附着的带血管的骨膜复合体将成为体内骨重建的良好和安全的材料之一。

To elucidate the sequential change of experimental osteogenesis in pores of periosteum-attached hydroxyapatite blocks, detailed microscopic observations and morphometric analysis of pores were carried out on the rabbit model. Neither bone nor adipose tissue was found in the pores of PHAB embedded in the muscle during the entire experimental period in the control group. By the contrast, newly formed bone tissue confirmed laminated architecture and depolarization was initially found at the margin of the pores present close to the face covered by periosteum of rectangular-shaped PHAB at 8th week, and this bone formation in pores extended to distal portion from the covered face. On the 12th week, adipose tissue was observed inside of the bone sloughing lined the pore. The morphometric analysis revealed a negative correlation between the percentage in area of bone in each pore and the distance from the face of PHAB attached periosteum.The result surmised that bone-sloughing formation lining pores and filled by adipose tissue with islets of erythroblasts is induced by differentiation of mesenchymal stem cell provided via periosteum attaching onto a face of PHAB. We wish to emphasize that complex of PHAB attached vascularized periosteum presented would become one of the favorable and secure material for bone reconstruction in vivo.