Ets family expression in cultured cells from oral tissue

为了阐明口腔疾病的发生机制，了解口腔组织中上皮与成纤维细胞的相互作用及其特征对口腔组织信号转导的影响是十分重要的。Ets因子是一类重要的转录调节因子，在造血、血管生成、器官发生、肿瘤发生和神经元连接规范中发挥关键作用。这些蛋白主要在造血细胞中表达，但也有一些在上皮细胞中表达。本研究研究了Ets基因家族、上皮特异性Ets转录因子家族成员-1、-2和-3 （ESE-1、-2和-3）在口腔黏膜癌源性细胞系HO-1-N-1上皮样细胞中的表达和调控。在没有兴奋剂的情况下，HO-1-N-1细胞本身表达了ESE-1、2和3 mRNA。IL-1α和IL-1β均能显著提高ESE-3 mRNA的表达，但对ESE-1和2 mRNA的表达无明显影响。Phorbol 12-肉豆蔻酸13-乙酸酯（PMA）也以剂量依赖的方式增加了ESE-3 mRNA的表达，PMA增强的ESE-3 mRNA表达被PKC抑制剂（bisindolylmale亚胺，BIS）和MEK1/2抑制剂（U0126）抑制。这些结果表明，ESE-3可能是炎症过程中的重要决定因素。此外，在HO-1-N-1细胞中，PKC激活剂增强的ESE-3 mRNA表达通过MEK1/2通路调控。

In order to clarify the mechanism of oral disease, it is quite important to know an interaction between epithelium and fibroblast and its characteristics on signal transduction in oral tissue. Ets factors constitute one important class of transcriptional regulators that play critical roles in hematopoiesis, angiogenesis, organogenesis, oncogenesis, and specification of neuronal connectivity. These proteins are expressed mainly in hematopoietic cells, however, some are also expressed in epithelial cells. The present study was investigated Ets gene family, Epithelium-specific ets transcription factor, family member-1,-2, and-3 (ESE-1,-2,and-3), expression and regulation in buccal mucosa carcinoma-derived cell line, HO-1-N-1,epithelial-like cell. HO-1-N-1 cell itself showed ESE-1,-2 and-3 mRNA expression in the absence of stimulant. IL-1α and IL-1β increased ESE-3 mRNA expression in a dose-dependent manner, however, they did not clearly alter ESE-1 and-2 mRNA expression. Phorbol 12-myristate 13-acetate (PMA) also increased ESE-3 mRNA expression in a dose-dependent manner, and ESE-3 mRNA expression enhanced by PMA was inhibited by PKC inhibitor (bisindolylmaleimide, BIS) and MEK1/2 inhibitor (U0126). These results suggest that ESE-3 might be an important determinant in an inflammation process. In addition, ESE-3 mRNA expression enhanced by PKC activator is regulated through MEK1/2 pathway in HO-1-N-1 cell.