The fundamental research of the direct pulp capping agent added a biocompatibility material

几丁质/壳聚糖作为一种有效的药物已广泛应用于医学和牙科的许多领域。然而，如果使用直接盖髓药物，我们报告它在早期产生严重的炎症。因此，在牙科临床应用前，有必要控制初始炎症反应。本研究对甲壳素/壳聚糖单体作为直接盖髓剂进行了评价。本实验选用8只Wistar雄性大鼠（体重约160g）的16个下门牙。在大鼠门牙预备好牙洞后，用尽可能小的尖牙探牙器暴露牙髓。暴露的牙髓表面分别用d -氨基葡萄糖或n -乙酰- d -氨基葡萄糖进行修饰。其余空腔用牙水泥填充。术后0、1、3、5 d处死大鼠灌注固定。用乙醇脱水后包埋在环氧树脂中。半薄切片用甲苯胺蓝染色进行光镜观察。目前的研究表明，直接用d -氨基葡萄糖覆盖牙髓可以诱导成纤维细胞增殖的快速愈合，而不会在早期产生严重的炎症。然而，在整个实验期间，n -乙酰- d -氨基葡萄糖直接盖髓时，观察到炎症细胞的浸润和坏死或退行性组织的面积。本研究表明，壳聚糖单体与甲壳素单体相比，即使在创面愈合的初始阶段，其生物相容性也非常稳定。

Chitin/chitosan has been used as an effective medicament on many fields of medicine and dentistry. However, in case of the use as direct pulp capping medicament, we reported that it produced severe inflammation on the early stage.Then, initial inflammation reactions are necessary to be controlled before clinical application in dentistry.The present study was undertaken to evaluate chitin/chitosan monomer for a direct pulp capping medicament. Sixteen lower incisors of 8 Wistar male rats(weight about 160g) were used for the present experiments. After cavity preparation in rat incisors, the pulp was exposed with a sharp dental explore as small as possible. The surface of the exposed pulp was dressed with either D-Glucosamine or N-Acetyl-D-Glucosamine. The rest of cavity was filled with dental cement.At 0, 1, 3 and 5 days postoperatively, rat were sacrificed in perfusion fixation. The block were dehydrated in ethanol and embedded in epoxy resin. Semithin sections were stained with toluidine blue for light microscopy. The present investigations indicate that direct pulp capping with D-Glucosamine induces a rapid healing with fibloblast proliferation without producing severe inflammation on the early stage. However, the inflammatory cell infiltration and the area of necrotic or degenerative tissue were observed all through the experiment period in case of direct pulp capping with N-Acetyl-D-Glucosamine. This study revealed that chitosan monomer extremely acts as a biocompatibly stable medicament even at the initial stage of wound healing in comparison with the application of chitin monomer.