Callus formation of membrous bone reconstruction using micro vascular endochondral bone

近年来，富含板状血浆(RPR)已在临床上用于促进骨再生和创面愈合。然而，目前还没有明确的研究报道PRP在制备后的活性。我们建立了一套从少量血液中安全生产RPR的方法，并对23名健康受试者的血液标本进行了不同条件的红外线抗凝剂和离心机分离，以探讨最佳浓缩条件。结果表明，以20%ACD-A液为抗凝剂时，800转/分和1300转/分两次离心法获得的PRP效果最好。通过血小板聚集反应和离心后PRP中转化生长因子-β的含量来证实其作用。

Recently, plate-rich plasma (RPR) has been used clinically to promote bone regeneration and wound healing. However, no study has clearly reported on the activity of PRP after preparation. We established a protocol for the safe production of RPR from small quantities of blood.Various conditions were used fir anticoagulants and centrifugal separation speed for blood sample obtained from 23 healthy subjects to investigate optimal concentration condition. Our results showed that when 20% ACD-A liquid was used as the anticoagulant, PRP was most efficiently obtained by double centrifugation at 800 r.p.m and 1300 r.p.m. The effective of PRP was confirmed by platelet aggregation reaction and the TGF-β content of PRP after centrifugation.