Investigation on the condition to achieve the best peripheral tissue reconstruction after maxillary alveolar distraction osteogenesis

为了探讨牵张成骨后实现最佳外周组织重建的条件，我们试图提取1。2.新生愈伤组织；老茧附近的牙齿长出的老茧我们的初步研究发现，血流需要很长时间才能恢复到原来的水平，而骨矿物质密度（BMD）在开颅100天后也没有恢复到原来的水平。因此，我们看到高压氧治疗（Hyperbaric Oxygen Therapy， HBO）的优势，临床上已被用于促进骨折的恢复，我们旨在研究高压氧治疗对比格犬血流量和骨密度恢复的影响。在6只成年beagle犬左上颚做10 mm骨缺损，然后制作含犬骨的骨段，以1mm/d的速度运输10天。延长后，对三只狗进行HBO治疗。采用激光多普勒血流仪监测犬牙髓血流100 d。牺牲后，每个上颚被解剖并使用pQCT（外周定量计算机断层扫描）测量骨密度。采用Mann-Whitney秩和检验对血流量和骨密度进行统计学分析。血流量恢复到原来的水平要快30天，hbo治疗犬的皮质骨密度更高。本研究提示高压氧可促进狗的愈合。关于牙齿向生成的愈伤组织中运动的研究，我们刚刚建立了实验系统。

To investigate the condition to achieve the best peripheral tissue reconstruction after distraction osteogenesis, we tried to extract the characteristics of 1. newly generated callus and 2. the callus after which nearby tooth has moved into. In our preliminary research, it is revealed that a long time was necessary for blood flow to recover to the original level, and the bone mineral density (BMD) did not get back to the original level even 100 days after distraction was initiated. Therefore, we saw the advantage of Hyperbaric Oxygen Therapy (HBO), which has been clinically used to facilitate the recovery of fractured bones, and aimed to investigate the influence of HBO on the recovery of blood flow and BMD using beagle dogs. The 10 mm bone defect was made in the left palate of 6 adult beagle dogs, then a bony segment including canine was made and transported at a rate of 1mm/day for 10 days. After the lengthening, HBO was administered to three dogs. Using laser Doppler flowmeter, blood flow of the canine pulp was monitored for 100 days. After the sacrifice, each palate was dissected and measured for BMD using pQCT (peripheral quantitative computed tomography). The blood flow and BMD were statistically analyzed using Mann-Whitney rank sum test. Blood flow recovered to the original level 30 days faster, the cortical BMD was higher in HBO-treated dogs. This study suggests that HBO is useful to facilitate the healing in DOG. In regard to the research on tooth movement into the generated callus, we have just established the experimental system of it.