Construction of Tissue Engineered Vascular Autograft and Heart Valve utilizing Biodegradable Scaffold

本研究采用犬骨髓单个核细胞（MN-BMCs）作为支架材料，将其接种于由聚乙交酯纤维和聚（1-丙交酯-co-ε-己内酯）海绵组成的可生物降解管状支架上，构建了组织工程化自体血管（TEVA）和组织工程化心脏瓣膜（TEHV）。在手术当天将该支架植入同一只狗的IVC中。另一方面，将体外培养的混合静脉细胞接种于聚羟基乙酸构建的生物可降解支架上，构建TEHV。植入后对TEVA和TEHV进行生物化学、生物力学和组织学分析，植入后6个月TEVA的生物化学性质和壁厚与自体IVC相似，对静脉压耐受良好，无钙化等问题。这些结果表明，TEVA和TEHV是具有功能性内皮细胞和生物力学性能的生物相容性材料，没有不良副作用。

This study demonstrates the evaluation of tissue-engineered vascular autografts (TEVAs) and Tissue Engineered Heart Valves (TEHV) constructed with autologous mononuclear-bone marrow cells (MN-BMCs) and a biodegradable scaffold using an experimental animal model.MN-BMCs were obtained from a dog and seeded onto a biodegradable tubular scaffold consisting of polyglycolide fiber and poly(1-lactide-co-ε-caprolactone) sponge. This scaffold was implanted in the IVC of the same dog on the day of surgery. On the other hanc, TEHV were constructed with mixed venous cells which were cultured in vitro followed by seeding onto the biodegradable scaffold which was constructed with polyglycolic acid. TEVAs and TEHV were analyzed biochemically, biomechanically and histologically after implantation.TEVAs had similar biochemical properties and wall thickness as native IVC at 6 months after implantation, and tolerated venous pressure well without any problems such as calcification. And TEHV tolerated RV pressure after implantation.These results indicate that TEVAs and TEHV are biocompatible material with functional endothelial cells and biomechanical properties without unwanted side-effects.