Fabrication of cell recognition device by the luminescent nanocrystalline silicon and development of the biomedical measurement system

发光半导体纳米粒子如硫化镉(Cd S)和硒化镉(Cd Se)在生物医学工程领域的应用已被广泛研究。然而，这些纳米材料在对生物体的安全性、生产成本、资源、环境影响和颗粒大小等方面存在一些问题。因此，人们有望开发出无害、价格适中、资源丰富、对环境影响小、粒径小的新型纳米材料。通过对纳米硅颗粒大小的控制，制备了从红光到蓝光连续发光的纳米硅颗粒，研究了纳米硅颗粒在动物静脉中的发光特性和流动条件。通过硅片/二氧化硅靶材的共溅射和高温后退火，在硅衬底上形成了尺寸为2.5 nm的纳米硅颗粒，嵌入氧化层中。The…然后，在氢氟酸水蒸气中对纳米硅颗粒周围更多的氧化层进行刻蚀，以有效地将许多颗粒提取到衬底表面。由于吸附在纳米硅颗粒表面的氢氟酸颗粒对生物体具有较高的毒性，样品经刻蚀处理后，在纯水中清洗。用超声振荡法将清洗后的纳米硅颗粒从衬底上抖落到生理盐水溶液中。然后，将含有纳米硅颗粒的NSS注射到动物体内，如大鼠和羔羊。利用光子多道分析仪可以很容易地检测脉状纳米硅颗粒的发光性质。光源为氙灯，带通滤光片波长为313 nm。当纳米硅颗粒在静脉中以1.3 mg的浓度注入时，它们稳定而平稳地流入通道。此外，在纳米硅颗粒在静脉中流动的条件下，它们还表现出强烈的红色发光，峰值位于720 nm。在活体中的冠状动脉、肠壁等不同位置也证实了良好的流动和红色发光。这些现象是由于形成了2.5 nm的小颗粒以及颗粒表面存在稳定的钝化膜所致。较少

Luminescent semiconductor nanoparticles such as cadmium sulfide (CdS) and cadmium selenide (CdSe) have been widely studied for application to biomedical engineering fields. These nanoparticle materials, however, have some problems for safety to living organism, production cost, resource, environmental influence and particle size. Therefore, it is expected to develop the new nanoparticle materials with some features of unharmfulness, moderate price, resourcefulness, low environmental influence and minimal particle size. We have been fabricated the nanocrystalline silicon (nc-Si) particles, which continuously emits from red light up to blue light by controlling the particle size.In this study, the luminescent property and flow condition of nc-Si particles in the vein of animal have been investigated. The nc-Si particles with a size of 2.5 nm embedding in an oxidized layer were formed on a Si substrate by co-sputtering of Si chips/silica targets and post-annealing at high temperature. The … More oxidized layer surrounding nc-Si particles, then, was etched in hydrofluoric (HF) acid steam to effectively extract the many particles onto the substrate surface. After etching treatment, the sample was rinsed in the pure water, because the HF acid particle adsorbed on the surface of nc-Si particle have the high toxicity for the living organism. The nc-Si particles after rinse treatment were shaken off from the substrate into the normal saline solution (NSS) by supersonic vibration treatment. After that, the NSS containing the nc-Si particles was injected into the vein of animal such as rat and lamb. The luminescent property of nc-Si particles in the vein was easily detected by using a photonic multichannel analyzer. A Xenon lamp was used as the light source with an optical bandpass filter of 313 nm. When the nc-Si particles were injected at the particle concentration of 1.3 mg in the vein, they stably and smoothly flowed into their channels. Moreover, they exhibited strong red luminescence with a peak at 720 nm under the flow condition of nc-Si particles in the vein. The good flow and red luminescence was also confirmed for the various positions such as a coronary artery and wall of the small intestine in the living organism. These phenomenon were achieved by the formation of particle with the small size of 2.5 nm and existence of stable passivation film on the particle surface. Less