Development of blood flow analysis system for total blood through micro-channel in biochip

为了解决生物芯片微通道模型中血浆流动与红细胞变形之间的耦合问题，提出了一种实用的二维、轴对称和三维浸没边界法计算方法。在浸没边界法中，将红细胞膜作为体力处理在流动方程中。因此，通过将结构分析程序融入代码高度调整的流动分析软件的用户子程序中，可以显著提高编程效率和计算速度。提出了这种方法，并通过对坦克踩踏运动和红细胞降落伞形变形的数值模拟，验证了该方法的有效性。假定红细胞轴对称变形的近似方法在某些情况下是有用的。然后，给出了膜…轴对称变形的基本方程显示了更多的红细胞，并对红细胞在微通道中的变形进行了数值模拟。将所得结果与二维结果进行了比较，并验证轴对称变形理论的适用性。为了验证计算结果，对红细胞作为毛细血管模型通过微通道阵列的行为和变形性进行了体外实验。观察了红细胞的变形和恢复过程，并进行了理论分析。通过对呼吸道基底膜的建模，得到合理的膜特性，阐明了在小时间尺度现象中，膜的粘性效应是必须考虑的，并在验证实验的基础上提出了膜的阻尼系数的估算方法。为了得到红细胞与通道壁的接触特性，计算了模型红细胞在微操作器中变形后的杨氏模数。此外，对MEMS制作的血液采集系统中的血液流动进行了观察，并对其适用性进行了检验。较少

Practical two-dimensional, axi-symmetric and three-dimensional calculation methods by means of the immersed boundary method were shown in order to solve the coupled problem between flow of blood plasma and deformation of red blood cell in the flow channel as a model of micro-channel in biochips. In the immersed boundary method, the membrane of red blood cell is treated as body force in the flow equation. Therefore, by incorporating the structural analysis program into user-subroutine of flow analysis software whose code is highly tuned, it is possible to increase efficiencies of making program and calculation speed remarkably. Such a method was proposed and the effectiveness of the method was shown by performing numerical simulations of the tank-treading motion and the parachute-shaped deformation of red blood cell. Approximation method assuming axi-symmetric deformation of red blood cell will be useful in some situations. Then, basic equations for axi-symmetric deformation of membrane … More of red blood cell were shown and numerical simulation of the deformation of red blood cell flowing in the micro-channel was performed. The results thus obtained were compared with two-dimensional results and applicability of the axi-symmetric deformation theory was checked. In order to validate the calculation results, in-vitro experiment was carried out on the behavior and the deformability of red blood cell passing through micro-channel array as a model of blood capillary. Deformation and recovery process of the red blood cell was observed and theoretical analysis was performed. Through modeling of basement membrane of the airway to obtain reasonable membrane characteristics, it was clarified that viscous effect of the membrane is necessary to take into account in small time-scale phenomena, and the estimation method of the damping coefficient of the membrane was proposed based on validation experiment. Young's modulus of modeled red blood cell was calculated which was deformed in the micro-manipulator in order to obtain contact characteristics of red blood cell with channel wall. Furthermore, blood flow in blood collecting system fabricated by MEMS was observed and the applicability was checked. Less