Effective use of the saccharide involved in drainage from paper manufacturing and cellulose powder producing processes.

通过有效利用纤维素类生物质制备细菌纤维素生产培养基。采用浓硫酸两段水解法水解造纸污泥中的废纸浆。考察了第一步和第二步水解的硫酸浓度，确定了适宜的水解条件。第一次用72%以上的硫酸处理，第二次在100℃下用5 ~ 25%的硫酸处理，纤维素几乎完全转化为葡萄糖。第二步水解的时间与硫酸浓度密切相关，由于纤维素粉产品是由漂白纸浆部分水解制得的，该过程的排水中含有大量有用的还原糖，但也含有硫酸，为了有效利用纤维素水解液中的葡萄糖和排水中的还原糖，通过电渗析检测与糖共存的硫酸的去除。从电渗析中获得的糖溶液用于制备培养基。

The culture medium for bacterial cellulose production was prepared by effective use of cellulosic biomass. The waste pulp in paper sludge was hydrolyzed by two-stage hydrolysis in concentrated sulfuric acid process. To get suitable conditions, the concentration of sulfuric acid were examined at the first and second hydrolysissatage, respectively. When treated with more 72% sulfuric acid at the first, with 5〜25% under 100℃ at the second, cellulose was converted to glucosse almost completely. The time of hydrolysis at the second was highly depend on the concentration of sulfuric acid used.Since cellulose powder products were prepared by the partial hyrolysis of bleached pulp with sulfuric acid, the drainage of the process includes a large quantity of useful reducing sugars, but it also contains sulfuric acid.In this study, for effective use of glucose in the cellulose hydrolyzate and reducing sugars in the drainage, the removal of the sulfuric acid coexisting with the saccharide was examined by means of electrodialysis. The saccharide solutions avairable from electrodialysis were used for the preparation of the culture medium.