Study of the extensively usable coronaviruses infrction model-mouse production.

猫传染性腹膜炎病毒（FIPV）是一种可感染猫的1组冠状病毒，根据其在体外的生长能力和与其它冠状病毒的抗原关系可分为两型。包括II型FIPV在内的一些组1冠状病毒使用猫氨肽酶N（fAPN）作为细胞表面受体。另一方面，有观点认为I型FIPV通过不同的受体进入靶细胞。为了分析I型和II型FIPV与fAPN之间的关系，我们使用大肠杆菌（E.coli）表达系统制备了重组fAPN，并使用重组fAPN作为抗原制备了抗fAPN的单克隆和多克隆抗体（MAb和PAb）。这些抗体与猫卡图斯全胎（fcwf-4）细胞反应。我们检测了这些抗体是否抑制病毒对fcwf-4细胞的感染。结果，抗fAPN多克隆抗体阻断两种类型的FIPV的感染，而单克隆抗体仅阻断II型FIPV。这些结果表明，fAPN负责两种类型的FIPV感染靶细胞。

Feline infectious peritonitis virus (FIPV), one of group 1 coronavirus infective to cats, is divided into two types on the basis of in vitro growth ability and antigenic relationship to other coronaviruses. Some group 1 coronaviruses including type II FIPV use feline aminopeptidase N (fAPN) as a cell surface receptor. On the other hand, there is an opinion that type I FIPV enters to target cells via a different receptor. To analyze a relationship between type I and II FIPV and fAPN, we produced recombinant fAPN using Escherichia coli (E.coli ) expression system and anti-fAPN monoclonal and polyclonal antibodies (MAbs and PAb) by use of the recombinant fAPN as antigen. These antibodies were reacted to Felis catus whole fetus (fcwf-4) cells. We examined whether these antibodies inhibit viral infection to fcwf-4 cells or not. In the result, Anti-fAPN polyclonal antibody blocked infection with both types of FIPV, while monoclonal antibodies blocked only type II FIPV. These results suggest that fAPN responsible for both types FIPV infection to target cells.