補助人工心臓下の虚血性心筋症に対する平滑筋細胞移植の効果

本研究采用同基因lewis大鼠。首先用6-0 Prolene结扎雌性大鼠左前降支，获得前路心肌梗死。术后复查超声心动图检查心功能。心肌梗死后4周，行超声心动图检查，若心肌梗死率达25%，则进入下一步。在全身麻醉下，取出梗死心脏作为供心，植入受者腹部，用9-0 Prolene将供者升主动脉、肺动脉与腹主动脉、下腔静脉吻合。t组同时移植平滑肌细胞，异位移植4周后切除心脏，用langendorff仪测定心功能，计数移植的雄性细胞，并做组织病理学检查。Langendorff研究中，T组心脏功能明显优于C组（对照组）。此外，男性平滑肌细胞移植到梗死心肌的比例约为15%。T组心功能好于C组的原因之一是T组的小动脉数量高于C组，对心功能有较好的影响。综上所述，梗死心肌细胞移植可有效改善心功能。这一概念可能应用于临床环境，例如患有心力衰竭的患者在平滑肌细胞移植后是否可以脱离LVAD植入。

Syngeneic Lwis rats were used for this study. First, left anterior descending artery was ligated using 6-0 Prolene to obtain anterior myocardial infarction in the female rats. After this procedure, echocardiography was repeated to examine cardiac function. 4 weeks after myocardial infarction, echocardiography was done, and if 25%of myocardium was infarcted, that rat went to next step. Under general anesthesia, infarcted hearts were extirpated as donor heart, which was then implanted into recipient abdomen, where ascending aorta and pulmonary artery of donor were anastomosed to abdominal aorta and inferior vena cava using 9-0 Prolene. Simultaneously,, smooth muscle cells were transplanted in group T. 4weeks after heterotopic transplantation, hearts were excised in order to measure cardiac function with langendorff apparatus, to count transplanted male cells, and to do histopathological examination.In Langendorff study, hearts in group T showed better cardiac function than those in group C (control) significantly. In addition, the percentage of male smooth muscle cells transplanted into infarcted myocardium was about 15%. One of the reasons why cardiac function in group T was better than that in group C was that the number of artrioles in group T was higher than that in group C, so it has good impact on cardiac function.In conclusion, cell transplantation in infarcted myocardium could be effective in terms of cardiac function. This concept might be applied to clinical settings, such as whether or not the patients who suffered from heart failure, can wean from LVAD implantation after smooth muscle cell transplantation.