Experimental study of tendon adhesion prevention by a biocompatible polymer hydrogel.

2-甲基丙烯酰氧乙基磷酰胆碱(MPC)聚合物是我们独创的生物相容性聚合物，其侧链由类似生物膜磷脂的磷酰胆碱组成。MPC水凝胶可由聚(MPC-co-甲基丙烯酸)(PMA)和聚(MPC-co-甲基丙烯酸丁酯)(PMB)的水溶液混合而成。本研究探讨了应用MPC水凝胶对肌腱粘连和愈合的影响。1.确定了合成MPC水凝胶的最佳条件。通过改变MPC水凝胶的分子组成、相对分子质量、疏水单元的结构，制备了各种凝胶，并在模拟体液和大鼠模型中进行了评价。所建立的最佳凝胶用于以下研究2-4.2。观察MPC水凝胶对肌腱愈合的影响。肌腱切断缝合后，用MPC水凝胶覆盖损伤部位，闭合伤口。通过大体、组织学和力学测试评价肌腱愈合情况。大体观察MPC组几乎没有或没有粘连。HE染色显示两组损伤肌腱间隙类似地被胶原纤维桥接。两组抗拉强度无明显差异。观察MPC水凝胶预防肌腱粘连的效果。相同方法后，通过光镜和力学测试评价肌腱粘连。结果显示，对照组肌腱粘连严重。相比之下，MPC组几乎没有或几乎没有粘连。MPC水凝胶覆盖肌腱缝合部位的润滑性评价：通过测量手术后足趾周期性屈曲后的肌腱漂移阻力，发现MPC水凝胶具有良好的润滑性能。结论：肌腱缝合后立即应用MPC水凝胶可以在不影响愈合的情况下防止粘连。

2-methacryloyloxyethyl phosphorylcholine(MPC) polymer is our original biocompatible polymer whose side chain is composed of phosphorylcholine that resembles phospholipids of biomembrane. The MPC hydrogel can be generated by mixing aqueous solutions of poly (MPC-co-methacrylic acid)(PMA) and poly(MPC-co-n-butyl methacrylate)(PMB). The present study investigated the effects of the MPC-hydrogel application on tendon adhesion and healing.1.Determination of the optimum condition for synthesis of MPC hydrogelVarious gels were generated by changing the molecular composition, molecular weight, structure of hydrophobic units, and evaluated in simulated body fluid and using rat model. The established optimal gel was used for the following studies 2-4.2. Investigation of the effects of MPC hydrogel for tendon healing..After a tendon was cut and sutured, the MPC hydrogel was applied to cover the injury site and the wound was closed.Tendon healing was evaluated by macroscopic, histological and mechanical testings.Macroscopic findings showed little or no adhesion was observed in the MPC group.HE staining showed that gaps of the injured tendon in both groups were similarly bridged by collagen fibers.There was no significant difference on tensile strength between two groups.3. Investigation of the effects of MPC hydrogel for prevention of tendon adhesion.After the same manner, tendon adhesion was evaluated by microscopic and mechanical testings.The findings showed severe adhesion in the control group. In contrast, little or no adhesion was observed in the MPC group.4. Evaluation of the lubrication property of MPC hydrogel covering a tendon suture site.By measuring of the tendon excursion resistance after cyclic flexion of the operated toe, the excellent lubrication property of MPC hydrogel was revealed.In conclusion, the MPC-hydrogel application immediately after the tendon suture may prevent the adhesion without impairing the healing.