The effects of transplantation of dopaminergic neuron induced from bone marrow stromal cells into amblyopia model through cerebrospinal fluid in rat

本研究对大鼠剥夺性弱视进行了分析。2005年，我们制作了大鼠弱视模型，将骨髓基质细胞（BMSc）分化为多巴胺能神经元细胞（DNc），并将细胞经脑脊液移植到大鼠弱视模型中。2006年，我们使用抗体阵列和PCR分析了这些模型。抗体阵列显示弱视脑组织中酪氨酸羟化酶（Th）表达上调，凋亡信号蛋白和MAP激酶信号系统表达下调。PCR研究发现，弱视视网膜中Th下调，弱视大脑和视网膜中凋亡信号蛋白和MAP激酶信号系统下调。在细胞移植组，PCR研究显示这些蛋白、神经丝和GFAP表达上调。上述结果提示，BMSc源性DNc移植大鼠弱视模型可有效上调Th、抗凋亡通路及神经元细胞存活。在未来，有必要检查细胞移植与左旋多巴给药的效果。

We showed the analysis of rat deprivation amblyopia in this research. In 2005, we made the amblyopia models, differentiation of bone marrow stromal cells (BMSc) to dopaminergic neuronal cells (DNc), and cell transplantation via cerebrospinal fluid to rat amblyopia models. In 2006, we analyzed these models using antibody array and PCR. Antibody array showed up-regulation of Tyrosine hydroxylase (Th) and down-regulation of signal proteins for apoptotic and MAP kinase signaling system in amblyopic brain. PCR study showed down-regulation of Th in amblyopic retina, and down-regulation signal proteins for apoptotic and MAP kinase signaling system in both amblyopic brain and retina. In the cell transplantation group, PCR study showed up-regulation of these proteins, neurofilament and GFAP. These results suggest that the BMSc derived DNc transplantation into rat amblyopia model is effective in up-regulation of Th, anti-apoptotic pathway and the survival of neuronal cells. In the future, there is a necessity for examining the effect of cell transplantation compared with the administration of Levodopa.