Analysis of keloid in an organotypic culture wound healing model

1.我们从耳垂瘢痕疙瘩中获得成纤维细胞，并制作真皮模型（收缩胶原凝胶），并制作模型系统以测量收缩过程及其一致性（振动阻抗）。将瘢痕疙瘩真皮模型与正常皮肤来源的成纤维细胞模型进行比较。收缩过程发生迅速，堆芯模型收缩更剧烈。通过向培养基中加入瘢痕疙瘩药物曲安西龙（与正常成纤维细胞，至于稠度，恒定）抑制收缩过程和稠度。在瘢痕疙瘩真皮模型中，早期加入曲安西龙的模型与正常真皮模型的一致性无显著性差异。另外，在培养第5天后，将曲安西龙在培养基中研磨，对培养第10天的稠度无显著性差异。我们证明，有一项工作可以抑制胶原凝胶收缩， ...更多信息 增加曲安西龙收缩胶原凝胶的稠度.采用瘢痕疙瘩真皮替代物（模型），建立瘢痕疙瘩创面愈合模型。对于瘢痕疙瘩伤口愈合模型，其显示了表皮层的重建，这与正常伤口愈合模型相似。免疫组化染色未显示一般分化标志物表现的显著差异。另外，我们用去除成纤维细胞的无结构真皮（脱细胞真皮基质）制作皮肤模型。在无结构真皮创面愈合模型中，表皮层形成不充分。创面愈合模型中表皮的重建同样不充分，尤其是基底层和基底上层的形成较差，向皮质层的分化是特征性的。对于表皮化，提示成纤维细胞的存在大于成纤维细胞属性。我们从神经纤维瘤的咖啡Au lait斑获得的成纤维细胞在皮肤模型中的色素水平增加，超过正常皮肤来源的成纤维细胞在皮肤模型中的色素水平。皮肤模型可用于分析表皮和真皮的细胞间干扰影响的分析系统。少

1. We obtained fibroblasts from ear lobe keloid and made a corium model (contracted collagen gel) and made the model system to measure the course of the contraction and its consistency (impedance by vibration). A keloid corium model was compared with a model by normal skin origin fibroblasts. The contraction process occurred rapidly and corium model was contracted harder. The contraction process and consistency were inhibited by addition to culture media of the triamcinolone which was drug of keloid (with normal fibroblasts, as for the consistency, constant). As for the consistency of the keloid corium model that we made by adding triamcinolone in culture media in the early phase, there was not the significant difference for a normal corium model. In addition, it did not show a significant difference to consistency of culture the tenth day by grinding triamcinolone in culture media after culture the fifth day. We proved that there was a work to inhibit that collagen gel shrink and to d … More ecrease the consistency of contracted collagen gel in triamcinolone.2. Using a keloid dermal substitute (model), we made a keloid wound healing model. As for the keloid wound healing model, it revealed reconstitution of the epidermis layer which was similar to a normal wound healing model. Immunostaining did not show the remarked difference for manifestation of a general differentiation marker. In addition, we made the skin model that we used structurelessness corium (Acellular Dermal Matrix) which removed fibroblasts from. In a wound healing model by structurelessness corium, the layer formation of epidermis was insufficient. And the reconstitution of epidermis in a wound healing model was insufficient likewise, especially the formation of basal and suprabasal layers were bad, and differentiation to a cornified layer was pathognomonic. For epidermization, it was suggested that fibroblastic presence was massive than fibroblastic attribute.3. Pigment level increased in a skin model by the fibroblasts which we obtained from cafe au lait spots of neurofibroma, more than in a skin model by fibroblasts of a normal skin origin. Skin model was useful for a analysis system to review cell-cell interactional influence of epidermis and corium. Less