Parallel bioreactor system

在可重现和可控的条件下平行培养微生物是微生物表征的重要组成部分。在我的工作组中，我们处理生物基材料和化学品生产过程和菌株的优化。重点主要是生物基和可生物降解的多糖，我们在体内工程中使用。通过这种方法，我们创造了许多不同的菌株变体，我们在可重现和可控的条件下检查它们的产物形成。由于新胞外多糖变体的化学结构改变，生产率也存在很大差异。由于多糖的生物合成高度依赖能量和氧气，许多这些变体只能在生物反应器中的最佳条件下生产。在下文中，我们描述了这些新的多糖变体的基本结构-功能关系，从而获得新功能的方法。在进一步的步骤中，进行生物合成途径的菌株特异性优化以提高生产率。使用的菌株组合包括革兰氏阴性和革兰氏阳性细菌，但也包括丝状真菌和微藻。对于微藻，在可重现的条件下在光生物反应器中培养是必不可少的，具有不同波长的靶向照明的可能性，以分析光强度和波长对生长和产物形成的影响。除了对微生物多糖的研究外，我们最近还在开发利用C1化合物的菌株，该化合物可从合成气等中生产。为此目的，我们使用例如甲醇芽孢杆菌，这是以前被认为是非常困难的遗传可及性。然而，基于我们开发的分子生物学工具（CRISPR-Cas），高效处理现在似乎是成功可行的。用于B的培养。需要50 °C的甲醇稳定温度，而不会从培养容器中蒸发，这会严重干扰产品和物料流的计算。所要求的平行生物反应器系统满足所有这些要求，并提供气体混合站，废气分析，稳定的温度控制和使用光棒的可能性，该设备可以在我们的实验室中进行所有必要的平行培养实验。工作组。

The parallel cultivation of microorganisms under reproducible and controllable conditions is an essential component for the characterization of microorganisms. In my working group we deal with the optimization of processes and strains for the production of biobased materials and chemicals. The focus is mainly on bio-based and biodegradable polysaccharides, for which we use in vivo engineering. By this approach, we create many different strain variants, which we examine for their product formation under reproducible and controllable conditions. Due to the modified chemical structures of the new exopolysaccharide variants, there are also strong differences in productivity. Due to the highly energy- and thus oxygen-dependent biosynthesis of the polysaccharides, many of these variants can only be produced under optimal conditions in bioreactors. In the following, we characterize the basic structure-functional relationships of these new polysaccharide variants, and thus derive approaches for new functionalities. In further steps, the strain-specific optimization of the biosynthesis pathways is carried out in order to increase productivity. The portfolio of strains used includes Gram-negative and Gram-positive bacteria, but also filamentous fungi and microalgae. For microalgae, cultivation in photobioreactors under reproducible conditions is essential, with the possibility of targeted illumination with different wavelengths, in order to analyze the influence of light intensity and wavelength on growth and product formation. In addition to research on microbial polysaccharides, we are recently working on the development of strains which utilize C1 compounds, which can be produced from e.g. synthesis gas. For this purpose, we use e.g. Bacillus methanolicus, which was previously considered to be very difficult to genetic accessibility. Based on molecular biology tools (CRISPR-Cas) developed by us, however, efficient processing now seems to be successfully feasible. For the cultivation of B. methanolicus stable temperatures of 50 °C are necessary, without evaporation from the cultivation vessels, which massively disturbs the accounting of the products and material flows.The requested parallel bioreactor system meets all these requirements and offers with a gas mixing station, exhaust gas analysis, stable temperature control and the possibility of using light rods a device that can serve all necessary parallel cultivation experiments in our working group.