Genetic manipulation of Clostridium sporogenes

该项目的总体目标是开发操作厌氧产孢子梭菌的遗传方法。具体地说，该项目的目标是操纵这种微生物，以便将编码将前药物转化为抗癌药物的酶的基因引入并稳定地保持在其染色体上。该项目的意义在于，生成的细菌和其他使用这些方法构建的细菌将能够测试它们作为治疗实体肿瘤的特定药物输送系统的能力。

The overall objective of this project is to develop genetic methods for the manipulation of the anaerobic bacterium Clostridium sporogenes. Specifically, the project aims to manipulate this microorganism so that genes encoding enzymes that convert prodrugs to anticancer drugs can be introduced and stably maintained on its chromosome. The significance of the project is that the resultant bacteria, and others constructed using these methods, will then be able to be tested for their ability to act as specific drug delivery systems for use in the treatment of solid tumours.