Molecular Analyses of Mechanisms of Pollination and Ferulzation

(1)芸苔属植物的自交不亲和。采用多种生化和分子生物学方法对油菜花粉提取物和花粉mRNA进行了分析，发现了花粉s -决定因子的候选分子。我们进一步分析了包括SLG和SRK的基因组克隆。并发现了一些基因，尤其是在花粉和花药中。我们成功地用FISH技术在油菜DNA纤维中可视化了SRK和SLO基因。(2)日本梨与相关植物的自交不亲和性我们克隆并测序了日本梨的S-RNase基因，并成功获得了S-RNase晶体，用于x射线分析，确定了S-RNase的分子结构。(3) Ipomea的自亲和性我们从野生Ipomea中分离到了一个编码柱头特异性蛋白的cDNA，并分析了其在柱头中的表达。该基因定位在距s位点1.2 cM处。(4)胚胎发生利用突变植株，我们已经鉴定出12个影响水稻早期胚胎发生的基因位点。本文分析了胡萝卜胚和不定胚早期表达的一个基因，并与拟南芥的一个同源基因进行了比较，在启动子区发现了一个顺式序列。

(1) Self-incompatibility of Brassica.We have analyzed pollen extract and pollen mRNA of Brassica campestris using various biochemical and molecular biological methods and found candidate molecules of the pollen S-determinant. Further we analyzed the genomic clone including SLG and SRK. and found several genes which would he expressed especially in pollen and anther. We have succeeded in visualization of SRK and SLO genes in the DNA fibets of Brassica rapa with FISH.(2) Self-incompatibility of Japanese pear and related plantsWe have cloned and sequenced S-RNase genes of Japanese pear and further we have succeeded in obtaing crystals of S-Rnase for X-ray analysis to determine the molecular structure of S-RNase.(3) Self-incompanbility of IpomeaWe have isolated a cDNA encoding a stigma-specific protein from wild species of Ipomea and analyzed its expression in the stigma. The gene was mapped at the distance of 1.2 cM from the S-locus.(4) EmbriogenesisWe have identified 12 loci affecting embryogenesis of rice at early stages using mutant plants. We have analyzed a gene expressed in embryo and adventitious embryo of carrot at a early stage and identified a cis sequence at the promoter region in comparison of a homologous gene of Arabidopsis.