Genome-wide study on pathogenesis of bacteria causing nosocomial infection

以金黄色葡萄球菌、粘质沙雷菌、铜绿假单胞菌为目标菌。对于鼠伤寒沙门氏金黄色葡萄球菌，我们描绘了基因集存在于S。金黄色葡萄球菌引起的某些疾病，并分析候选基因，以检测或预测特定的毒力。金黄色。对于鼠伤寒沙门氏marcescens，我们通过动物实验筛选毒力相关基因，并调查参与人血清抗性的因素（基因）。对于铜绿假单胞菌，我们研究了与人血清抗性有关的因素（基因）。分析了MDRP克隆的特征和耐药机制。

We employed Staphylococcus aureus, Serratia marcescens, Pseudomonas aeruginosa as target agent causing nosocomial infection. For S. aureus, we delineated gene sets present in S. aureus causing certain diseases and analyzed candidate genes to detect or foresee specific virulence of S. aureus. For S. marcescens, we screened for virulence-related genes by animal experiment and investigated for factors (genes) involved in human serum resistance. For P. aeruginosa, we investigated for factors (genes) involved in human serum resistance. We also analyzed characteristics and antimicrobial resistance mechanism of MDRP clones.