Deciphering mechanisms of synaptic remodeling at the morphological and functional levels

通过这个程序，我们确定了几个新的CREB调节激酶。此外，我们表明，立即早期基因弧是一个真正的CREB靶基因，并阐明了转录机制参与活性依赖性弧基因激活。此外，我们的特点是必不可少的作用，CaMKI亚型的树突和轴突的生长在围产期发展的神经元回路。最后，PSD和突触前活动区支架蛋白（如PSD-95，RIM 1和Shank）的新的调节模式进行了研究，重点是它们与肌动蛋白细胞骨架重塑的联系。与此同时，我们开发了新的协议来可视化和操纵突触的形成和神经元的形态重塑。

Through this program, we identified several new CREB-regulatory kinases. Furthermore, we showed that the immediate early gene Arc is a bona fide CREB target gene, and elucidated the transcriptional mechanisms implicated in activity-dependent Arc gene activation. Additionally, we characterized the essential roles of CaMKI isoforms in the growth of dendrites and axons during the perinatal development of neuronal circuits. Finally, novel modes of regulation of PSD and presynaptic active zone scaffolding proteins (such as PSD-95, RIM1 and Shank) were investigated, with emphasis on their link with actin cytoskeletal remodeling. In parallel, we developed new protocols to visualize and manipulate synapse formation and morphological remodeling of neurons.