Research of cell detachment behavior from nano-biomaterial surfaces

利用聚n -异丙基丙烯酰胺修饰温度响应表面的细胞片工程已被应用于再生医学。细胞的附着/剥离特性在很大程度上取决于这些聚合物层的厚度。然而，人们对水条件下由相变引起的温度响应聚合物层的厚度知之甚少。本文采用原子力显微镜、x射线光电子能谱和蛋白质吸附法对不同厚度的热响应表面进行了表征。此外，我们还讨论了热敏聚合物表面的细胞附着/脱离特性的厚度依赖性。

Cell sheet engineering using poly(N-isopropylacrylamide)-modified temperature responsive surfaces has been applied to regenerative medicine. Cell attachment/detachment properties strongly depend on the thickness of these polymer layers. However, little is known about the thickness of the temperature responsive polymer layer arising from phase transition under aqueous conditions. In this paper, we characterize different thicknesses of thermoresponsive surfaces using atomic force microscopy, X-ray photoelectron spectroscopy and protein adsorption assay. Furthermore, we discuss the thickness dependency of cell attachment/detachment properties of thermoresponsive polymeric surfaces.