Analysis on regulatory factors and functional mechanisms for the initiator protein of the chromosomal replication

染色体DNA复制的起始是调节细胞增殖的关键反应。大肠杆菌DnaA蛋白的活性形式启动染色体复制，而非活性形式不启动。这项研究首次揭示了染色体上的特定DNA序列（称为DARS）直接激活DnaA。此外，它揭示了分子机制的刺激的起始反应的DnaA结合蛋白DiaA和在启动后失活的DnaA的Hda蛋白。

Initiation of the chromosomal DNA replication is a crucial reaction that regulates the cell proliferation. The active form of Escherichia coli DnaA protein initiates the chromosomal replication, whereas the inactive form does not. This study revealed for the first time that specific DNA sequences (termed DARS) on the chromosome directly activate DnaA. Moreover, it revealed molecular mechanisms in stimulation for the initiation reaction by the DnaA-binding protein DiaA and in post-initiational inactivation of DnaA by Hda protein.