High-sensitive biochemical gas sensor based on the gas-biomolecule interaction

研制了一种以NADH为电子受体的生物传感器。生物传感器测量底物的存在作为NADH的荧光。为了提高NADH对甲醛的敏感性，对其进行了化学扩增研究。醛脱氢酶在NAD+存在下氧化甲醛产生甲酸和NADH。我们共固定化甲酸脱氢酶，用甲酸再生NADH。因此，该传感器对甲醛的灵敏度为亚ppb级。并利用光催化剂TiO2进行了实样品测试。该传感器成功地利用光催化反应的作用实时跟踪了甲醛的变化。

A biosensor using NADH as an electron acceptor was developed. The biosensor measures the presence of the substrate as fluorescence of NADH. In order to enhance the sensitivity to formaldehyde, chemical amplification of NADH was investigated. Aldehyde dehydrogenase produces formic acid and NADH by oxidizing formaldehyde under the presence of NAD+. We co-immobilized formic acid dehydrogenase to re-produce NADH using formic acid. Consequently, the sensor showed sub-ppb level sensitivity to forimaldehyde. Also, real-sample test using photocatalystic TiO2 was carried out. The sensor successfully traced the change of formaldehyde by the effect of photocatalystic reaction in real-time.