Systems biology of AKT signaling

在细胞信号转导中，外部刺激中的信息被编码为信号活动的时间模式，然而，对时间编码机制的研究很少。在本研究中，我们根据实验结果对PC12细胞中依赖于表皮生长因子(EGF)的Akt通路进行了建模，并发现参与细胞生长的Akt通路是如何将上游信号的时间信息特异性地传递到下游的。此外，我们对一个简单的生化反应进行了理论分析，发现瞬时峰值幅度的信号传递效率随负调控强度的增加而衰减，并发现细胞可以通过改变负调控因子的表达水平来降低信号传递效率来控制下游的敏感性。

In cellular signal transduction, information in an external stimulus is coded as temporal patterns of signaling activities; however, the temporal coding mechanism has been poorly investigated. In this study, we modeled the epidermal growth factor (EGF)-dependent Akt pathway in PC12 cells based on experimental results, and found how the Akt pathway, which is involved in cell growth, specifically transfers temporal information of upstream signals to downstream. In addition, we theoretically analyzed a simple biochemical reaction and found that signal transfer efficiency of transient peak amplitude attenuates depending on the strength of negative regulation, and found that cells can control downstream sensitivity through attenuation of signal transfer efficiency by changing the expression level of negative regulators.