Studies on the assembly of super-molecular complex by synthetic approach

大多数蛋白质通过形成亚基复合体来表达功能。然而，对细胞中复合体的形成过程了解甚少。本项目采用纯化翻译因子组成的无细胞翻译系统PURE系统，在试管中重构了复杂的形成过程。结果，我们成功地合成了ATP合成酶，ATP合成酶是由22个多肽和3个多肽组成的转座子在细胞膜上形成的。在30S核糖体亚基生物合成相关因子的存在下，也成功实现了16SrRNA和20种核糖体蛋白的30S小亚基的高效重建。

Most of proteins are able to express function by forming a complex of subunit.However, the formation process of a complex in the cell is only poorly understood. In this project, using a cel-free translation system constituted with purified translation factors, PURE system, complex formation processes were reconstructed in the test-tube. As a result, we succeeded in the synthesis of the ATP synthase, which is formed from22 polypeptides and translocon composed of three polypeptides onto the cell membrane. the reconstruction of small subunit of the ribosome (30S) with high efficiency was also successfully achieved form a 16SrRNA and of 20 ribosomal proteins in the presence of the factors involved in biosynthesis of 30S ribosome subunit.