confocal laserscanning microscope

这项研究由德国<s:1>贝克大学（UzL）生物医学光学研究所（BMO）进行，研究重点是新的光学成像方法，这些方法是根据诊断应用而开发的。对于创新的基于光的治疗和诊断程序的发展，细胞过程变得越来越重要。由于目前研究所不可能对亚细胞动态过程进行常规的高分辨率成像，因此需要一台具有3个激发波长和2个检测通道的现代共聚焦激光扫描显微镜（cLSM）。该仪器将大力支持六个BMO研究小组、UzL的外部合作伙伴以及石勒苏益格-荷尔斯泰因大学医学中心（UKSH）的临床项目合作伙伴的科学工作。一方面，它将有助于新的光学成像技术（拉曼显微镜，SLIDE显微镜和相干断层扫描或显微镜）的发展，另一方面，它将用于研究蛋白质和细胞的光学操作以及眼科光学诊断和治疗方法的细胞机制。具体的应用领域是，例如，研究亚细胞分辨率的光活化剂在细胞内的运输，或研究热疗后细胞培养中RPE细胞的功能变化。该设备在BMO大楼的一个专用的、可集中访问的房间内操作，并由合格人员监督。

The research at the Institute of Biomedical Optics (BMO) at the University of Lübeck (UzL), focuses on new optical imaging methods, which are developed with regard to diagnostic applications.For the development of innovative light-based therapeutic and diagnostic procedures, cellular processes are becoming increasingly important. Since there is currently no possibility at the institute for routine, high-resolution imaging of subcellular dynamic processes, a modern confocal laser scanning microscope (cLSM) with 3 excitation wavelengths and 2 detection channels should be acquired.The instrument will significantly support the scientific work of six BMO research groups, external cooperation partners of the UzL and also clinical project partners at the University Medical Center Schleswig-Holstein (UKSH). On one hand, it will contribute to the development of new optical imaging techniques (Raman microscopy, SLIDE microscopy and coherence tomography or microscopy), and on the other hand, it will be used to investigate the optical manipulation of proteins and cells and the cellular mechanisms of optical diagnostic and therapeutic methods in ophthalmology. Concrete fields of application are, for example, investigations of the intracellular transport of photoactivatable agents with subcellular resolution or the investigation of functional changes of RPE cells in cell cultures after hyperthermia. The device is operated in a dedicated, centrally accessible room in the BMO building and is supervised by qualified personnel.