The research of pathogenesis, development's mechanism and treatment of Pars Tensa Cholesteatoma.

张力部（PT）中耳炎行软骨鼓室成形术，可长期防止术后鼓膜回缩，听力改善良好。中耳积液与PT-中耳炎有一定关系。认为上皮下成纤维细胞参与了中耳瘤上皮的活动和中耳瘤的扩展。上皮和上皮下组织的相互作用在中耳炎的发生发展中起重要作用。通过动物实验，将兔鼻粘膜上皮细胞片置于温度敏感性培养板中培养，并将其移植到中耳，证实了良好的粘膜再生。再生的粘膜在组织学和功能（中耳腔压力测量）方面显示出几乎正常的状态。我们证实Caspase-14（1.170）与上皮细胞的分化有关，特异性地发生在中耳炎上皮细胞中。提示Caspase-14启动子有助于治疗（持续性中耳乳突瘤上皮消失）中耳乳突瘤。

Cartilage tympanoplasty for Pars tensa(PT) cholesteatoma prevent postoperative retraction ofn tympanic membrane in the long term, and it was found that the hearing improvement was good. There are some relations between Otisis media with effusion and PT-cholesteatoma. It was thought that fibroblasts in subepithelial layer were involved in activity of cholesteatoma epithelium and cholesteatoma extension. The interaction of epithelium and the subepithelial tissue play ley role for development of cholesteatoma. We cultured a rabbit nasal mucosa epithelial cells sheet in temperature-responsive culture plate by animal experiment, and we transplanted it to the middle ear, and good mucosa regenaration was confirmed. Regenerated mucosa shows almost normal conditions with a histologically and functionally(pressure measurement of the middle ear cavity). Weconfirm that Caspase-14 where 1.170 is associated with for the differentiation of epithelial cells specifically develops in cholesteatoma epithelial cells. It is suggested that Caspase-14 promoter helped treatment (disappearance of the persistentcholesteatoma epithelium) of the middle ear cholesteatoma.