Utilization of viscoelastic substrates to study actin/MTs coordination and subsequent mechanobiological functionalities

ECM是改变细胞反应的多种机械和生化信号的来源。使用生物材料破译这种复杂性将增加我们对这些独特的细胞行为的理解在生病和健康conditions. Although粘弹性作为一个机械cue，我专注于ECM浓度作为一个化学线索，调查的MT和肌动球蛋白串扰。在这项工作中，cRGD作为一种化学线索，引起肌动蛋白分布的改变，这将改变肌动蛋白-MTs串扰（初步结果在一次会议上发表）。在联合研究中，光活化表面被用于研究重力矢量对集体细胞迁移和3D细胞结构的影响。这些允许远程诱导细胞迁移。细胞对重力矢量的反转导致肌动球蛋白细胞骨架系统的重塑，改变了3D簇结构和迁移表型（初步结果在会议上发表，论文Accepted STAM 2023）。在另一项联合研究中，研究了细胞与表皮生长因子变体功能化的金纳米颗粒的相互作用。阐明了特定GNP-EGF变体的选择性细胞毒活性的机制（发表在Analytical Science 2023）。另一项研究，我们使用AFM研究了细胞骨架对各种环境刺激的反应，我们测量了各种条件下的细胞骨架应力分布（初步结果在会议上发表，提交论文）

ECM is the source of diversified mechanical and biochemical cues that alters cellular responses. Deciphering this complexity using biomaterials would increase our understanding of these unique cellular behaviors in ailing and health conditions.In addition to the viscoelastic properties as a mechanical cue, I focused on the ECM concentrations as a chemical cue to investigate the MTs and actomyosin crosstalk. In this work, cRGD as a chemical cue causes an alteration in the actin distribution, and this would alter Actin-MTs crosstalk (Preliminary results was presented at a conference).In joint research, the photoactivatable surfaces were used to investigate the effect of gravity vector on collective cell migration and 3D cellular architectures. These allowed the remote induction of cellular migration. The inversion of cells against the gravity vector resulted in the remodeling of the actomyosin cytoskeletal system that alters the 3D cluster architecture and migratory phenotype (Preliminary results were presented at a conference, paper Accepted STAM 2023).In another joint research, the interactions of the cells with gold nanoparticles functionalized with epidermal growth factor variants were studied. The mechanism for the selective cytotoxic activity of a specific GNP-EGF variant was illustrated (Published in Analytical Science 2023).Another research, we investigated the cytoskeletal response to various environmental stimuli using AFM, we measured the cytoskeletal stress distribution under various conditions (Preliminary results were presented at a conference, Paper submitted)