Identification and Characterization of Novel Components Involved in the Export of Ribosomes from the Nucleolus into the Cytoplasm

核糖体从细胞核向细胞质输出的机制尚不清楚。由于核糖体的生物发生途径极其复杂，与tRNA等其他物质的核输出相比，核糖体的核输出可能更为复杂。使用60S亚基报告基因（Rpl25p-eGFP），我们分离了20个温度敏感核糖体输出（rix）突变体。这些rix突变体的野生型基因已通过互补克隆出来。这表明大量具有不同亚细胞位置和功能的成分参与了核糖体的输出。在我们未来的研究中，我们将对这些Rix因子进行表征，这将对核糖体组装和随后转运到细胞质之间的耦合提供分子洞察力。

The mechanism of ribosomal export from the nucleus to the cytoplasm is not understood. As compared to the nuclear export of other cargoes (e.g. tRNA), nuclear export of ribosomes is likely to be more complicated, in view of an extremely complex biogenesis pathway of ribosomes. Using a 60S subunit reporter (Rpl25p-eGFP), we have isolated 20 temperature-sensitive ribosomal export (rix) mutants. The wild-type genes of these rix mutants have been cloned by complementation. This revealed that a large number of components with distinct subcellular location and function are involved in ribosome export. In our future research, we will characterize these Rix factors, which should give a molecular insight in the coupling between ribosome assembly and subsequent transport to the cytoplasm.