Live-Cell Epifluorescence Microscope

在菲利普斯大学马尔堡病毒学研究所，几个小组研究影响人类健康的高致病性病毒的感染、复制和致病性的分子机制，包括埃博拉病毒和马尔堡病毒、冠状病毒（SARS、MERS和SARS- cov -2）、甲型流感病毒、不同的黄病毒（如登革热、寨卡病毒和西尼罗河病毒）、拉沙病毒和尼帕病毒。该研究所拥有可用于研究这些病毒的最高生物安全水平的实验室。该研究所的许多小组利用高端显微镜方法研究病毒复制和对受感染宿主细胞的影响，以确定对病毒进入、复制和退出至关重要的宿主细胞机制，并阐明受感染细胞的病理改变。除了分析病毒感染细胞的固定样本外，工作组还对病毒复制室的形成和子代病毒粒子的组装和释放的动力学感兴趣。此外，与不同宿主细胞器的相互作用也是研究的另一个重点。为了研究这些过程，该研究所在BSL3和BSL4设施中分别配备了一台新的尼康Ti2-E和一台徕卡DM6000 FS活细胞显微镜。然而，BSL2设施中的活细胞显微镜是一台已有13年历史的尼康TE-200E仪器。由于它的年龄，仪器经常出现故障，相机不是最先进的状态，维修备件不再可用。为了继续对这些重要的人类病原体进行非常成功的研究，必须尽快更换仪器。

At the Institute of Virology of the Philipps-University Marburg several groups investigate molecular mechanisms of infection, replication, and pathogenicity of highly pathogenic viruses that affect human health, including Ebola and Marburg virus, coronaviruses (SARS, MERS, and SARS-CoV-2), influenza A virus, different flaviviruses (e.g. Dengue, Zika, and West Nile), Lassa, and Nipah virus. The institute has laboratories up to the highest biosafety level that allow the study of these viruses. Many groups in the Institute utilize, among other techniques, high-end microscopy methods to study viral replication and the effects on the infected host cells to identify host cell machineries that are critical for virus entry, replication, and egress and to elucidate pathological alterations in infected cells. Beside the analysis of fixed samples of virus-infected cells, the working groups are interested in the dynamics of the formation of virus replication compartments and assembly and release of progeny virions. In addition, the interaction with different host cell organelles is another main focus of research. To study those processes, the institute has live-cell microscopes that are available in the BSL3 and BSL4 facilities, a new Nikon Ti2-E and a Leica DM6000 FS, respectively. However, the live cell microscope in the BSL2 facility is a 13-year-old Nikon TE-200E instrument. Due to its age the instrument frequently fails, the cameras are not state of the art and spare parts for repair are no longer available. To continue the highly successful research on these important human pathogens, the instrument has to be replaced as soon as possible.