Production of human renin in microorganism and its application to medicine.

1)根据人肾素前体的互补序列推断其初级结构。预测序列由406个氨基酸组成，前段和前段分别携带20个和46个氨基酸。2)基于天冬氨酸蛋白酶的整体折叠非常相似的假设，构建了人肾素的三维模型。作为参考，我们使用了青霉素。3)从Charon 4A人类基因组文库中分离到人肾素基因，并对其进行了鉴定。该基因跨越约11.7千碱基，由10个外显子和9个内含子组成，这些内含子位于酶的可变表面环的点上。4)在大肠杆菌trp启动子-算子的转录控制下，在表达载体中产生了人prorenin。转化后的大肠杆菌细胞作为杂交蛋白的一部分表达了高水平的prorenin（占细胞总蛋白的30%）。

1) The primary structure of human renin precursor was deduced from its complementary sequence. The predicted sequence consists of 406 amino acids with a pre- and a prosegment carrying 20 and 46 amino acids, respectively.2) A three-dimensional model of human renin has been constructed based on the assumption that the overall folding of the aspartyl proteases is very similar. As a reference, we used penicillopepsin.3) The human renin gene was isolated from a Charon 4A human genomic library and characterized. The gene spans about 11.7 kilobases and consists of 10 exons and 9 introns that map at points that could be variable surface loops of the enzyme.4) Human prorenin was produded under the transcriptional control of the E. coli trp promoter-operator in the expression vector. The transformed E. coli cell expressed high levels (30% of total cell protein) of prorenin as part of a hybrid protein.