Androgenic regulation of acyl-CoA dehydrogenase expression

观察了哈德氏地金仓鼠脂质组成的性别差异。雄性腺体中含有直链脂肪酸，雌性腺体中还含有支链脂肪酸。这些差异是由雄激素调节酰基辅酶a脱氢酶引起的；异戊酰辅酶a脱氢酶（IVD）和短/支链酰基辅酶a脱氢酶（SBCAD）是支链脂肪酸在腺体中形成的关键酶。在本研究中，我们发现这两种酶的活性受雄激素的调节。这种调控是组织特异性的，发生在基因表达的步骤中。我们还发现，雄激素在哈德氏腺中诱导雄激素受体（AR），并且这种诱导先于IVD和SBCAD的诱导。我们还测定了仓鼠AR cDNA的序列，发现了负责人类AR自调节的同源区域。

Sexual differences are observed in the lipid composition of golden hamster Harderian land. Male gland contains straight chain fatty acids, but that of female additionally contains branched chain ones. These differences were generated by the androgenic regulation of acyl-CoA dehydrogenases ; isovaleryl-CoA dehydrogenase (IVD) and short/branched chain acyl-CoA dehydrogenase (SBCAD), which are the key enzymes for the formation of branched chain fatty acids in the gland.In this study, we showed that the activity of these two enzymes were regulated by androgen. This regulation was tissue-specific and occurred at the step of gene expression. We also found that androgen receptors (AR) were induced by androgen in Harderian gland, and this induction was preceded the induction of IVD and SBCAD.We also determined the sequence of hamster AR cDNA and found homologous regions which were responsible for the auto-regulation of human AR.