Control of acyl-CoA dehydrogenase expression by androgen.

金黄地鼠哈氏腺分泌大量的脂类，主要成分为1-烷基-2，3-二酰基甘油（ADG），其组成存在明显的性别差异。雄性腺体含有直链脂肪酸，而雌性腺体还含有支链脂肪酸。我们发现这些差异是由雄激素调节的酰基辅酶A脱氢酶，这是形成支链脂肪酸的关键酶。薄层色谱法分析日增重，发现了性别差异，即雄性日增重谱由3个斑点组成，雌性由2个斑点组成。利用该腺体的特性，我们通过分析该腺体分泌液的ADG谱来监测雄激素和抗雄激素物质的效果。将去势的雄性仓鼠（手术后2周）皮下注射睾酮（含或不含抗雄激素，如氟替卡松）。我们用玻璃毛细管从每只动物获得约1 μ l的腺液，持续2周。经去势处理后，TLC上的ADG曲线变为雌性型。替吉奥治疗使其特征恢复为男性类型，具有时间和剂量依赖性。然而，当额外注射抗雄激素时，这些变化被消除。抑制作用也依赖于抗雄激素的剂量。这些结果清楚地表明，我们可以很容易地评估雄激素和抗雄激素材料的影响，使用动物非侵入性。

The Harderian gland of golden hamster secretes copious lipids consisting mainly of 1-alkyl-2, 3-diacylglycerol(ADG), the composition of which shows marked sexual differences. Male gland contains straight chain fatty acids, while female gland additionally contains branched chain ones. We found these differences were caused by the androgenic regulation of acyl-CoA dehydrogenases which are the key enzymes for the formation of branched chain fatty acids. The sexual differences were detectable by analyzing ADG on TLC.Namely, ADG profile was composed of 3 spots in male while 2 spots in female. Utilizing the characteristics of this gland, we monitored the effects of androgenic and anti-androgenic materials by analyzing ADG profile of secreted fluid from the gland. Castrated male hamsters(2 weeks after operation)were subcutaneously injected with testosterone with or without anti-androgens such as flutamide. We obtained about 1 μ1 of gland fluid with a glass capillary from each animal for 2 weeks. ADG profile on TLC changed into female type by castration. Testosterone treatment returned he profile to male type time- and dose -dependently. However these changes were abolished when anti-androgens were additionally injected. The inhibition was also dependent on the dose of anti-androgens. These results clearly show that we can easily evaluate the effects of androgenic and anti-androgenic materials using animals noninvasively.