Novel Biosensors Using Electropolymerized Film

本研究的目的是通过对电聚合膜生物传感器件的研究，积累对新型生物传感器的基础知识，并建立新的功能接口设计。特别是在本研究中，将电聚合钝化聚合物膜应用于生物传感器的制备，为生物传感器的应用提供了新的尝试，明确了电聚合钝化聚吡咯膜与聚离子络合物(PLC)成膜法的结合是酶在功能膜中掺入和包埋的有效途径。此外，还阐明了该传感器适用于流动注射分析。在此基础上，设计并制作了一种新型肌酐生物传感器。利用电聚合钝化聚吡咯和PLC进行肌酸传感的体系，对于酶反应引起pH变化的传感体系具有很好的适用性。此外，利用这些结果对PLC的分子筛分作用进行了研究。揭示了电聚合钝化聚合物生物传感器和PLC生物传感器的基本现象，为新型传感系统的研究提供了新的思路。

This study was done aiming to pile the basic knowledge on the novel biosensors and to establish the design of new functional interface with the investigation of biosensing devices using electropolymerized films. Especially, in this study, an electropolymerized inactive polymer film was applied to fabricate the biosensors, and it is a new attempt to use for the sensors.From the study, the combination of electropolymerized inactive polypyrrole film and film formation method of polyion complex (PlC) is cleared to be an effective way to incorporation and entrapment of enzyme in the functional film. In addition, it was clarified that this sensor is applicable to the flow injection analysis. Based on these results, a new biosensor for creatinine was designed and fabricated. The system using electropolymerized inactive polypyrrole and PlC for a creatinine sensing is well applicable to the sensing system whose enzyme reaction causes a pH change. In addition, with these results, molecular sieving effects of PlC were studied. This enables us to make a variation of sensitivity for the sensors with the PlC structure.In conclusion, the basic phenomena of biosensors using electropolymerized inactive polymer and PlC were revealed and new approach for novel sensing system was proposed.