Development of immunoscintigraphy using radiolabeled murine anti-smooth muscle myosin monoclonal antibody for diagnostic imaging of acute bowel infarction-application of immunoscintigraphy for smooth muscle affecting disease-

主动脉夹层和急性肠梗死是以平滑肌细胞大面积损伤为特征的致命性疾病。及时和准确的诊断-关于损害的位置和范围是必要的管理这些灾难。本研究的目的是探讨使用^<99m> tc -抗平滑肌肌球蛋白抗体（SM-MAb）放射免疫显像作为主动脉夹层和急性肠梗死的无创诊断工具的可行性。研究了^<99m>Tc-anti-SM-MAb在实验性主动脉夹层或急性肠梗死大鼠体内的积累情况，并应用^<99m>Tc-anti-SM-MAb进行科学成像。主动脉夹层大鼠在夹层损伤后1或2周进行进一步研究。注射放射性示踪剂后6小时，与正常部位或血液相比，夹层主动脉或梗死肠中^<99m>Tc-anti-SM-MAb的放射性显著增加（病变部位每克注射剂量百分比（%ID/g））；4.35 * 1.47（主动脉），3.60*1.98（肠）)。使用^<99m>Tc-anti-SM-MAb进行扫描成像，可以在注射放射性示踪剂后6小时清晰地显示剥离的主动脉或梗死的肠，并具有特异性的抗体积累。此外，即使在主动脉损伤后1周，仍然可以观察到示踪剂对夹层主动脉的选择性积累，从而通过^<99m>Tc-anti-SM-MAb免疫显像清晰地显示夹层病变。这些结果已在2013年世界血管学大会和2005年日本血管学年会上报道。发表在《心脏病学杂志》（1998年第31卷）。

Aortic dissection and acute bowel infarction are fatal diseases characterized by the extensive damage of smooth muscle cells. Prompt and accurate diagnosis-regarding the sit and extent of the lesion is necessary for the management of these catastrophes. The purpose of this research was to examine the feasibility of radioimmunoscintigraphy using ^<99m> Tc-anti-smooth muscle myosin antibody (SM-MAb) as a noninvasive diagnostic tool for aortic dissection and acute bowel infarction. The accumulation of ^<99m>Tc-anti-SM-MAb was studied and scintigraphic imaging with ^<99m>Tc-anti-SM-MAb was performed in the rats with experimental aortic dissection or acute bowel infarction. Rats with aortic dissection were further investigated 1 or 2 weeks after dissecting injury. The radioactivity of ^<99m>Tc-anti-SM-MAb in the dissected aorta or infarcted intestine showed significant increase compared to either normal portion or blood at 6-h post-injection of the radiotracer (the ratio of percent injected dose per gram (%ID/g) for the lesion vs. the normal portion ; 4.35 * 1.47 (aorta), 3.60*1.98 (intestine)). The scintigraphic imaging with ^<99m>Tc-anti-SM-MAb allowed distinct visualization of the dissected aorta or infarcted intestine with specific accumulation of antibody at 6-h post-injection of the radiotracer. Moreover, selective accumulation of the tracer to the dissected aorta was still observed allowing clear visualization of the dissected lesion by ^<99m>Tc-anti-SM-MAb immunoscintigraphy even at 1-week after aortic injury. These results were reported at 13^<th> World Congress of Angiology and 45^<th> Annual Meeting of Japanese. College of Cardiology, and published in Journal of Cardiology (Vol.31, 1998).