Basic research on spinal cord perfusion on acute spinal cord injury

用脊髓诱发电位(SCEP)评价脊髓损伤后的脊髓功能，分析人工脑脊液(ACSF)对脊髓损伤后脊髓功能的影响。这只动物在胸中水平进行了椎板切除，并被固定在一个立体定位框架内。通过椎板切除插入导管电极以记录SCEP。用微执行器低速压迫脊髓，直到SCEP波幅略低于初始波幅的10%。5只动物行十二指肠切开加常温充氧ACSF脊髓灌流4h。伤后4h，再灌流动物SCEP波幅平均恢复到43.6%。相比之下，在对照动物中几乎没有发现任何潜力。两组差异有统计学意义，提示急性脊髓损伤后局部灌流是一种有效的功能恢复治疗方法，其机制可能与抑制早期继发性神经元损伤有关。需要更长时间的观察和更大的动物进行进一步的研究。

The purpose of this study is to evaluate the spinal cord function using spinal cord evoked potential (SCEP) and to analyze the effect of perfusion with artificial cerebrospinal fluid (aCSF) after the spinal cord injury.Ten adult Wister rats were anesthetized and immobilized with halothane and suxamethonium chloride. The animal had a laminectomy at mid thoracic level and was fixed in a stereotaxic frame. Catheter electrodes were inserted through the laminectomy for SCEP recording. Spinal cord was injured by low velocity compression with microactuator until SCEP amplitude became just below 10% of the initial amplitude. In 5 animals, durotomy and spinal cord perfusion with normothermic oxygenated aCSF was performed for 4 hours. The other 5 animals were remained as non-treated controls.4 hours after the injury, perfused animals showed the recovery of SCEP amplitude to 43.6% in average. In contrast, almost no potential was identified at this time in the control animals. The difference of the two groups was statistically significant, and this suggests that the local perfusion after acute spinal cord injury is a useful treatment for the functional recovery probably due to the suppression of secondary neuronal damage in early stage. Further study with longer observation and larger animals will be required.