Experimental study for elucidation of the onset mechanism in the phantom pain

我们试图建立包括幻肢痛在内的周围神经性疼痛的实验动物模型。麻醉下，切除大鼠一侧（SD和无毛）正中神经、尺神经和桡神经（1组）或拔出（2组）。在接下来的5个月里，两组用von Frey毛测试前爪对机械刺激的敏感性。在整个测试期间，记录每只大鼠的一般行为。在手臂去神经后，50%（组1）和20%（组2）的自体切开术发生爆炸。无毛大鼠皮肤溃疡发生率均为50%（两组）。机械刺激试验各组均未产生机械异常性疼痛。神经切开术后第7天，背根神经节（C4 ~ Th1）部分大、中细胞表现出神经肽Y （NPY）样免疫反应性（IR），并在随后的5个月观察到数量明显增加。2组大鼠星状神经节内NPY-IR细胞数量增加。烟碱腺嘌呤二核苷酸磷酸染色细胞强吗？在接下来的7天内，观察组1的SG中NADPH-d的变化。正常大鼠SG未见NADPH-d染色细胞。我们认为，对包括幻肢痛在内的神经性疼痛的研究必须采用不同的种类和科。

We attempted to develop an experimental animal model for peripheral neuropathic pain including the phantom pain. Under anesthesia, median, ulnar and raidiar nerves of one side of the rat (SD and hairless) were cut (group 1) or pull out (group 2). Sensitivity of the fore paw to mechanical stimulation was tested with von Frey hair for the next 5 months in two groups. The general behavior of each rat was noted during the entire test period.Following arm denervation, autotomy in 50% (group 1) and 20% (group 2) exploded. In the hairless rat, the ulcer of the skin was observed in 50% (both group). The test with mechanical stimulation in all groups did not produce a mechanical allodynia. Seven days following axotomy of the nerves, some large and medium sized cells in the dorsal root ganglia (C4〜Th1) displayed neuropeptide Y (NPY)-like immunoreactivity (IR), and marked increases in the numbers were observed for the next 5 months. The number of NPY-IR cells in the stellate ganglion (SG) increased in the group 2. A few strong staining cells for the nicotinamaide adenin dinucleotide phosphate ? Diaphorase (NADPH-d) were observed in the SG of the group 1 for the next 7 days. NADPH-d staining cells were not detected in the normal rat SG.We concluded that the study of the neuropathic pain including the phantom pain must be done using different species and families.