Novel siderophore reductases in Gram-negative bacteria

铁载体途径不仅是细菌和真菌获取必需离子Fe3+的途径，也为新型抗菌化合物的开发提供了可能。我们的最终长期目标是了解革兰氏阴性病原体不同蛋白质家族Fe3+/铁载体减少的分子机制，并随后利用这一知识探索新型铁载体-抗生素结合物的设计。在这个项目中，我们重点研究FoxB(COG3182)家族的内膜还原酶的还原机制。我们试图了解来自革兰氏阴性病原体的FoxB家族的膜还原酶如何从其铁载体底物中释放亚铁。此外，我们还将确定铁铁载体还原的电子给体，并确定与FoxB的络合结构。

Ferric-siderophore pathways are not only a way for bacteria and fungi to acquire the essential ion Fe3+ but also offer a possibility for the development of novel antimicrobial compounds. Our ultimate long-term goal is to understand the molecular mechanisms of Fe3+/siderophore reduction for various protein families from Gram-negative pathogens and to subsequently use this knowledge to explore the design of novel siderophore-antibiotic conjugates. Within this project, we focus on the reduction mechanism of inner membrane reductases of the FoxB (COG3182) family. We seek to understand how membrane reductases of the FoxB family from Gram-negative pathogens function to release ferrous iron from their siderophore substrates. In addition, we will identify the electron donor for the Fe-siderophore reduction and determine the complex structure with FoxB.