The interrelationship between each gut organ modulated by gastrointestinal hormones

我们的研究项目包括以下几类。(1)建立慢性胃瘘犬实验模型，在清醒状态下连续收集胰胃液。(2)血浆胆囊收缩素（CCK）对胰腺外分泌和胆囊收缩有重要生理作用，建立了特异、灵敏的CCK放射免疫分析方法。(3)研究胃肠激素如胃泌素释放肽（GRP）、神经介肽C、肽YY（PYY）、肽组氨酸异亮氨酸（PHI）对胰腺外分泌和血浆胃肠激素（包括CCK）水平的影响，以阐明这些肽的生物活性和生理重要性。(4)对患有慢性胃瘘的狗在十二指肠内滴注脂肪或食物刺激后同时测定胰腺外分泌和内源性释放的CCK，以确定CCK是否具有真正的生理意义。(5)人内源性CCK及消化道手术切除后胃肠激素释放变化的观察(6)胃肠激素产生细胞包括CCK的免疫组织化学定位与胃肠激素组织浓度的放射免疫测定的比较研究。

Our research project consists of the following categories.(1) Construction of the experimental model of the dog with chronic and gastric fistulas which allows us continuous collection of pancreatic and gastric juice in the conscious state.(2) Establishment of the specific and sensitive radioimmunoassay for plasma cholecystokinin (CCK) that plays a physiologically important role for exocrine pancreatic secretion and gallbladder contraction.(3) Study on the effect of gastrointestinal hormones, such as Gastrin Releasing Peptide (GRP), Neuromedin C, Peptide YY (PYY), Peptide Histidine Isoleucine (PHI), on exocrine pancreatic secretion and plasma levels of gastrointestinal hormones including CCK to clarify the biological activity and physiological importance of these peptides.(4) Simultaneous measurment of exocrine pancreatic secretion and endogeneouslly released CCK on dogs with chronic and gastric fistulas following introduodenal instillation of fat or food stimulation to determine if CCK has true physiological importance.(5) Observation on the endogeneouslly released CCK in human and on the changes of the release of gastrointestinal hormones following resection of gut organs in the digestive surgery.(6) Comparative study of the immunohistochemical localization of the gastrointestinal hormone producing cells including CCK and radioimmunoassay measurement of tissue concentrations of gastrointestinal hormones.