Study for the physiological functions of cytoskeleton-related calmodulin-binding proteins.

钙离子是细胞内最重要的信使之一，参与细胞收缩、内吞、胞吐和细胞分裂等多种功能。钙信号是由肌钙蛋白C和钙调蛋白等钙受体蛋白介导的。细胞骨架是蛋白质细丝的三维细胞内网络，是这些细胞功能的分子基础。我们一直在研究这些功能的钙依赖调控，并发现了一组钙调素结合蛋白，它们也与细胞骨架相互作用。在本研究中，我们研究了细胞骨架相互作用的钙调素结合蛋白介导的钙调素依赖的细胞骨架调控机制：1.钙调素和钙调素对MAPs-肌动蛋白相互作用的调节。与ConA受体封顶相关的钙调蛋白在脾T淋巴细胞上的再分布。TPA诱导3T3细胞肌动蛋白和钙调素的重组。钙依赖的调节…更多的是膜下细胞骨架。蛋白质4.1-钙调蛋白复合体对血影蛋白-肌动蛋白相互作用的调节。胞磷脂，一种35 kDa的细胞骨架相互作用和钙调蛋白结合蛋白的功能。一种新的84/82 kDa钙调蛋白结合蛋白--突触素I.8的鉴定牛肾上腺髓质80 kDa钙离子依赖性肌动蛋白切断蛋白的纯化F-肌动蛋白的交联是由钙调蛋白聚集体引起的，而不是由它的二聚体引起的。肌球蛋白轻链多重磷酸化与肌球蛋白ATPase活性的关系。钙调蛋白对原肌球蛋白增强的肌球蛋白ATPase活性的调节。肾上腺髓质肌动球蛋白中肌动蛋白-肌球蛋白相互作用的双重调节。动脉平滑肌钙调蛋白和肌球蛋白轻链激酶的特性。-caldesmon35 kDa功能片段的分离。原肌球蛋白在肌动蛋白-细丝素相互作用中的作用。钙调素对树体收缩的调节作用。-和-钙调蛋白在平滑肌细胞表型转换过程中的表达。较少

Ca^<2+> is one of the most important intracelluar messenger in many cellular functions including contraction, endocytosis, exocytosis and cell division. Ca^<2+> signals are mediated by Ca^<2+>-receptive proteins such as troponin C and calmodulin. Cytoskeleton is the three-dimensional intracelluar meshwork of protein filaments which are molecular bases of these celluar functions. We have been studing the Ca^<2+>-dependent regulation of these functions, and have discovered a group of calmodulin-bindig proteins which also interact with cytoskeleton. In this study, we have investigated the Ca^<2+>-calmodulin dependent regulatory mechanism of cytoskeleton mediated by the cytoskeleton-interacting calmodulin-binding proteins:1. Regulation of MAPs-actin interaction by Ca^<2+> and calmodulin.2. Redistribution of caldesmon associated with Con A receptor capping on splenic T-lymphocytes.3. Reorganization of actin and calspectin in 3T3 cells induced by TPA treatment.4. Ca^<2+>-dependent regulation … More of subemembranous cytoskeleton.5. Regulation of spectrin-actin interaction by protein 4.1-calmodulin complex.6. Function of cytosynalin, a 35 kDa cytoskeleton-interacting and calmodulin-binding protein.7. Identification of a new 84/82 kDa calmodulin-binding protein as synapsin I.8. Purification of an 80 kDa Ca^<2+>-dependent actin-severing protein from bovein adrenal medulla.9. Crosslinking of F-actin caused by caldesmon aggregates, not by its dimers.10. Relationship of the multiple phosphorylation of myosin light chain and myosin ATPase activity.11. Regulation of the tropomyosin-enhanced myosin ATPase activity by caldesmon.12. Dual regulation of the actin-myosin interaction in adrenal medullary actomyosin.13. Characterization of caldesmon and myosin light chain kinase from arterial smooth muscle.14. Isolation of the 35 kDa functional fragment of -caldesmon.15. Role of tropomyosin in the actin-filamin interaction.16. Regulation of the tree-dimentional contraction by caldesmon.17. Expression of - and -caldesmons during phenotype chage of smooth muscle cells. Less