Studies on functional difference of blood vessels and sympathetic nerve mechanisms of them

本课题对交感神经支配血管的特性和特异性进行了研究。1.不同血管中儿茶酚胺含量存在差异。2.观察各血管内源性儿茶酚胺含量与外源性儿茶酚胺摄取的相关性。3.兔肺动脉内有高水平的多巴胺。外源性多巴胺的摄取和神经末梢的释放。这些结果提示动脉可能有多巴胺能神经支配。4.兔腹主动脉和下腔静脉肾上腺素水平较高，血管内存在肾上腺素合成酶。5.肾动、静脉中肾上腺素水平较高，可认为是从血流中摄取。6.研究心钠素(ANP)、尾加压素I(UI)等血管活性多肽对血管的作用。(1)肾动脉对ANP最为敏感，ANP的血管扩张作用可能是通过抑制平滑肌细胞的钙偶联收缩机制实现的。(2)肠系膜动脉对尿失禁最为敏感。在自发性高血压大鼠中，观察到随着高血压的发展，乌拉地平的血管扩张作用减弱，这种减弱可能是通过刺激内源性前列腺素合成来实现的。提示红景天的血管扩张作用可能是通过抑制钙离子内流来实现的。7.研究了衰老对大鼠血管内药物反应的影响。此外，还研究了随着年龄的变化，特别是水和盐的代谢。8.尼古丁处理一周后，血管对去甲肾上腺素的反应减弱。

In this project, the characteristics of the blood vessels and specificity of sympathetic nerve innervated them were studied. 1. There were differences on catecholamine contents among the blood vessels. 2. Correlation between endogenous catecholamine contents and uptake of exogenous catecholamines in each vessel was observed. 3. High level of dopamine in the rabbit pulmonary artery was found. Uptake of exogenous dopamine and release of it from nerve endings were shown. These results suggested possible dopaminergic innervation in the artery. 4. High level of adrenaline in rabbit abdominal aorta and inferior vena cava was found and the presence of adrenaline synthetase in the vessels was shown. 5. High level of adrenaline in the renal artery and vein was found and it was considered as uptake of it from blood stream. 6.Effects of vasoactive peptides such as atrial natriuretic peptide (ANP) and urotensin I (UI) on blood vessels were studied. (1) Renal artery was the most sensitive to ANP and vasodilatory effects of ANP was suggested to be mediated through the inhibition of calcium coupling contractile mechanism in the smooth muscle cells. (2) Mesenteric artery was the most sensitive to UI. Reduction of vasodilatory effect of UI in the spontaneously hypertensive rats with the development of hypertension was observed and the reduction was considered to be mediated through the stimulation of endogenous prostaglandins synthesis. Vasodilatory effect of UI was suggested to be mediated through the inhibition of calcium entry into the smooth muscle cells. 7. Effects of aging on the drug responses in the rat blood vessels were studied. Furthermore, changes with aging were studied with special reference to water and salt metabolism. 8. Reduction of the response of the blood vessels to noradrenaline by one week treatment of nicotine was observed.