Structure and the cellular location of the novel phosphonoglycosphingolipids in the nervous tissues of Aplysia

在二维薄层色谱中，Aaplasia的每个组织都显示出该组织特有的膦酰鞘脂模式。FGL-IIa和FGL-IIb对神经纤维具有特异性，FGL-V（G-13）似乎特异性地集中在神经组织中。采用三种柱层析方法从黑尾蝮蛇（Aaplasiakurodai）神经组织中分离得到三种新的膦酰鞘糖脂（FLG-IIa，FGL-IIb和FGL-V），并对其结构进行了表征。FGL-IIa是具有由葡萄糖、半乳糖和半乳糖胺组成的寡糖链的单磷酸糖鞘脂。FGL-IIb也是具有岩藻糖、葡萄糖、半乳糖和半乳糖胺作为寡糖部分的单磷酸糖鞘脂。FGL-V（G-13）是一种由葡萄糖、半乳糖、半乳糖胺和4-O-甲基葡萄糖胺组成的寡糖链的二磷酸鞘糖脂。二维薄层层析结合免疫放射自显影使用抗血清和<^（125）I> -IG，F（ab '）2，揭示了九个免疫反应糖脂提取物的神经纤维。用抗血清和HRP标记的抗兔IgG进行免疫组化，在神经组织中观察到明显而强烈的染色。皮肤和肌肉组织中的神经纤维也被染色。

On two-dimensional TLC, each tissue of Aplysia showed a pattern of phosphonoglycosphingolipids characteristic to the tissue. FGL-IIa and FGL-IIb were specific to the nerve fiber and FGL-V (G-13) was seemed to be specifically concentrated in the nervous tissues. Three novel phosphonoglycosphingolipids (FLG-IIa, FGL-IIb and FGL-V) were isolated from the nervous tissues of Aplysia kurodai, using three systems of silicic acid column chromatography and characterized. FGL-IIa was monophosphonoglycosphingolipid having an oligosaccharide chain consisting of glucose, galactose and galactosamine. FGL-IIb was also monophosphonoglycosphingolipid having fucose, glucose, galactose and galactosamine as an oligosaccharide moiety. FGL-V (G-13) seemed to be diphosphonoglycosphingolipid having an oligosaccharide chain consisting of glucose, galactose, galactosamine and 4-0-methylglucosamine.Antisera against FGL-IIa and FGL-IIb mixture were raised in rabbits. Two-dimensional thin-layer chromatography combined with immunoautoradiography using the antisera and <^(125)I> -Ig,F(ab')2, revealed nine immunoreactive glycolipids in extracts from the nerve fiber. By immunohistochemistry with the antisera and HRP-labelled antirabbit IgG, distinct and strong staining was observed in the nerovus tissue. Nerve fibers in the skin and muscle tissue were also stained.