Evaluation of chemotherapeutic effect upon 203 GL glioma in mice as studied by p-31-NMR spectroscopy.

采用P-31核磁共振和流式细胞术评价化疗对小鼠胶质瘤的作用。我们发现，低于LD-50剂量的ACNUor tegafur在24或48小时后导致无机磷酸盐(Pi)/磷酸肌酸（PCr）和磷酸同质酯(PME)/磷酸肌酸（PCr）的比例部分抑制，尽管这些比例通常随着肿瘤的生长而增加。体内胶质瘤的流式细胞分析显示，在治疗后24-48小时，含有四倍体DNA的细胞通过G-2-M阻滞发生积累，但这种变化发生的时间略晚于Pi/pcr比值。另一方面，给药后8天观察组织学变化。因此，体内p -31核磁共振光谱可以检测到化疗药物给药后24-48小时肿瘤代谢途径的变化。

The effect of chemotherapy against glioma in mouse was evaluated by P-31 NMR spectroscopy and flow cytometry. We found that administration of ACNUor tegafur at a dose less than LD-50 resulted in the partial suppression of the ratio of inorganic phosphate (Pi)/phosphocreatine (PCr) and phosphomonoester (PME)/creatine phosphate (PCr) after 24 or 48 hrs although these ratios are usually increased together with growth of tumors. Flow cytometric analysis of glioma in vivo showed an accumulation in cells containing tetraploid DNA by G-2-M block in 24-48 hrs after treatment However, the change occurred at a period slightly later than that of Pi/pcr ratio. On the other hand, histological change was noted at 8 days after administration. Hence, it is concluded that in vivo P-31-NMR spectroscopy can detect a change of metabolic pathways in tumors as early as 24-48 hr after administration of chemotherapeutic agents.