Taxonomical and ecological study of the gall disease bacteria isolated from the trees used for dyeing of Oshima and Kumejima tsumugi textiles.

在日本冲绳和鹿儿岛地区发现了一种新的细菌病，在每一个树干或树枝上形成了一棵新的细菌病（Rhaphiolepis umbellate Makino）树木。年轻女孩的表面相对光滑，浅绿色，但它们变得明亮，浅棕色到深棕色。从这些球中分离的病理性细菌是一根糖负性的棒，有1根到几根极菌、有氧和代谢化的葡萄糖氧化物。在以下试验中获得了阴性结果：kovacs氧化酶、levan生产、马铃薯软rot、esculin和淀粉的水解、arginine dihydrolase、H_2S的生产、硝酸减少、KCN抑制、吲哚生产、凝胶素纤维化、甲基红和Voges-Proskauyer试验和在41 ° C和4 ° C的生长。积极的结果被发现与这样的测试如碱性、烟草超敏感反应、吐温80的羟基化，以及从蔗糖和荧光颜料生产中减少物质。用有机化合物作为碳的单一来源被利用：核糖、葡萄糖、D-甘露糖、D-木糖、果糖、糖醇、D-山梨醇、甘露醇、柠檬酸、糖酸、L-马来酸和L-、中邻苯二甲酸。以下是未使用的：蔗糖、D-、L-花生糖、L-rhamnose、Dk-半乳糖、乳糖、麦芽糖、D-纤维素、melibiose、D-三氟醚、二次蛋白、葡萄糖原、麦芽糖、淀粉、氨基酚、腺苷醇、双西醇、水杨酸、丙二醇、乳酸、L-Valine、L-精氨酸<beta>和丙氨酸。对Sharinbai病原体的宿主范围进行的Inoculation测试是根据属于44个家族的105种树种进行的。该疾病的发展只被确认为Rhaphiolepis umbellata，而不是其他树木，从这些结果中，Sharinbai pathogen被确认为一个新的pathovar，Syringae。P. syringae pv. rhaphiolepidis Pv. 11月，不久将被提议为ATCC、NCPPB、PDDCC和应变RU 2将被设计为病理型应变。

A new bacterial disease with gall formation on either trunks or twigs was found on Sharinbai(Rhaphiolepis umbellate Makino) trees growing in Okinawa and Kagoshima Prefecture, Japan. The surface of young galls was relatively smooth and light-green in color, but they became rough and light-brown to dark-brown with age. A pathogenic bacterium isolated from these galls was gram-negative rod with 1 to several polar flagella, aerobic and metabolized glucose oxidatively. Negative results were obtained with the tests such as kovacs oxidase, levan production, potato soft rot, hydrolysis of esculin and starch, arginine dihydrolase, production of H_2S, nitrate reduction, KCN inhibition, indole production, gelatin liguefaction, methyl-red and Voges-Proskauyer tests and growth at 41゜C and 4゜C. Positive results were obtained with the tests such as catalase, tobacco hypersensitive reaction, hydrolysis of Tween 80, reducing substances from sucrose and fluorescent pigment production. Following organic compounds were utilized as a sole source of carbon : ribose, glucose, D-mannose, D-xylose, fructose, glycerol, D-sorbitol, mannitol, citrate, succinate, L-malate and L-, meso-tartrate. Followings were not utilized : sucrose, D-,L-arabinose, L-rhamnose, Dk-galactose, lactose, maltose, D-cellobiose, melibiose, D-trehalose, dextrin,glycogen, maltose, starch, inulin, inositol, adonitol, dulcitol, salicin, malonate, lactate, L-Valine, L-Arginine and <beta>-Alanine. Inoculation tests for the host range of the Sharinbai pathogen were conducted on trees of 105 species belonging to 44 families. The disease development was confirmed only on Rhaphiolepis umbellata, but not on other trees, From these results, the Sharinbai pathogen was identified as a new pathovar of Pseudomonas syringae. The name of P. syringae pv. rhaphiolepidis Pv. nov. will be soon proposed to ATCC, NCPPB, PDDCC and the strain RU 2 will be designated as the pathotype strain.