Immunohistochemical study on the rabbit cerebellum in early postnatal periods

分析了兔小脑对一种针对成年兔嗅球产生的单克隆抗体11-4G12 （MAb 4G12）的免疫染色模式。在产后早期，MAb 4G12能强烈标记前叶的浦肯野细胞，而MAb能轻微标记后叶的浦肯野细胞。前者属于胞周枝晶阶段，后者属于主枝晶阶段。前后叶的形态学特征和免疫染色强度有很大差异。上述免疫染色强度的区域差异也在胚胎第25天观察到，此时两叶的浦肯野细胞形态尚不成熟。因此，我们得出结论，对MAb 4G12免疫反应的区域差异不是由于浦肯野细胞成熟的差异。提示兔小脑浦肯野细胞在发育过程中存在分子异质性。免疫印迹分析显示，抗MAb 4G12的表位估计分子量为26 kd单位。

Immunostaining patterns of the cerebellum toward a monoclonal antibody, 11-4G12 (MAb 4G12), produced against the adult rabbit olfactory bulb were analysed in baby rabbits and rabbit fetuses. In the early postnatal periods, MAb 4G12 labeled intensely Purkinje cells in the anterior lobe whereas the MAb labeled slightly Purkinje cells in the posterior lobe. The former belonged to a perisomatic dendrite stage whereas the latter did to a main dndrite stage. There are great differences in morphological characteristics and immunostaining intensities between the anterior and posterior lobes.The abovementioned regional differences in immunostaing intensity also were observed at embryonic day 25 in which Purkinje cells in both lobes have an immature morphology. Thus, it is concluded that the regional difference in immunoreaction to MAb 4G12 is not due to difference in Purkinje cell maturation. This suggests that Purkinje cells of the rabbit cerebellum during development are segregated by molecular heterogenuiety. From immunobloting analyses, an epitope against MAb 4G12 had estimated molecular weights of 26 kd units.